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RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer

Circadian rhythm is an autoregulatory rhythm, which is sustained by various mechanisms. The nucleocytoplasmic shuttling of BMAL1 is essential for CLOCK translocation between cytoplasm and nucleus and maintenance of the correct pace of the circadian clock. Here we showed that RAE1 and NUP98 can promo...

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Autores principales: Zheng, Xulei, Zhao, Xu, Zhang, Yingying, Tan, Hao, Qiu, Bojun, Ma, Tengjiao, Zeng, Jiarong, Tao, Dachang, Liu, Yunqiang, Lu, Yilu, Ma, Yongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347605/
https://www.ncbi.nlm.nih.gov/pubmed/30683868
http://dx.doi.org/10.1038/s41419-019-1346-2
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author Zheng, Xulei
Zhao, Xu
Zhang, Yingying
Tan, Hao
Qiu, Bojun
Ma, Tengjiao
Zeng, Jiarong
Tao, Dachang
Liu, Yunqiang
Lu, Yilu
Ma, Yongxin
author_facet Zheng, Xulei
Zhao, Xu
Zhang, Yingying
Tan, Hao
Qiu, Bojun
Ma, Tengjiao
Zeng, Jiarong
Tao, Dachang
Liu, Yunqiang
Lu, Yilu
Ma, Yongxin
author_sort Zheng, Xulei
collection PubMed
description Circadian rhythm is an autoregulatory rhythm, which is sustained by various mechanisms. The nucleocytoplasmic shuttling of BMAL1 is essential for CLOCK translocation between cytoplasm and nucleus and maintenance of the correct pace of the circadian clock. Here we showed that RAE1 and NUP98 can promote the degradation of BMAL1 and CLOCK. Knockdown of RAE1 and NUP98 suppressed BMAL1 shuttling, leading to cytoplasm accumulation of CLOCK. Furthermore, Chip assay showed that knockdown of RAE1 and NUP98 can enhance the interaction between CLOCK: BMAL1 and E-box region in the promoters of Per2 and Cry1 while reducing its transcription activation activity. Our present study firstly revealed that RAE1 and NUP98 are critical regulators for BMAL1 shuttling.
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spelling pubmed-63476052019-01-28 RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer Zheng, Xulei Zhao, Xu Zhang, Yingying Tan, Hao Qiu, Bojun Ma, Tengjiao Zeng, Jiarong Tao, Dachang Liu, Yunqiang Lu, Yilu Ma, Yongxin Cell Death Dis Article Circadian rhythm is an autoregulatory rhythm, which is sustained by various mechanisms. The nucleocytoplasmic shuttling of BMAL1 is essential for CLOCK translocation between cytoplasm and nucleus and maintenance of the correct pace of the circadian clock. Here we showed that RAE1 and NUP98 can promote the degradation of BMAL1 and CLOCK. Knockdown of RAE1 and NUP98 suppressed BMAL1 shuttling, leading to cytoplasm accumulation of CLOCK. Furthermore, Chip assay showed that knockdown of RAE1 and NUP98 can enhance the interaction between CLOCK: BMAL1 and E-box region in the promoters of Per2 and Cry1 while reducing its transcription activation activity. Our present study firstly revealed that RAE1 and NUP98 are critical regulators for BMAL1 shuttling. Nature Publishing Group UK 2019-01-25 /pmc/articles/PMC6347605/ /pubmed/30683868 http://dx.doi.org/10.1038/s41419-019-1346-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zheng, Xulei
Zhao, Xu
Zhang, Yingying
Tan, Hao
Qiu, Bojun
Ma, Tengjiao
Zeng, Jiarong
Tao, Dachang
Liu, Yunqiang
Lu, Yilu
Ma, Yongxin
RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title_full RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title_fullStr RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title_full_unstemmed RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title_short RAE1 promotes BMAL1 shuttling and regulates degradation and activity of CLOCK: BMAL1 heterodimer
title_sort rae1 promotes bmal1 shuttling and regulates degradation and activity of clock: bmal1 heterodimer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347605/
https://www.ncbi.nlm.nih.gov/pubmed/30683868
http://dx.doi.org/10.1038/s41419-019-1346-2
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