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IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development

Primary cilia and intraflagellar transport (IFT) proteins control a wide variety of processes during tissue development and homeostasis. However, their role in regulation of stem cell properties during tooth development remains elusive. Here, we revealed that dental pulp stem cells (DPSCs) express I...

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Autores principales: Yuan, Xue, Cao, Xu, Yang, Shuying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347632/
https://www.ncbi.nlm.nih.gov/pubmed/30683845
http://dx.doi.org/10.1038/s41419-018-0951-9
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author Yuan, Xue
Cao, Xu
Yang, Shuying
author_facet Yuan, Xue
Cao, Xu
Yang, Shuying
author_sort Yuan, Xue
collection PubMed
description Primary cilia and intraflagellar transport (IFT) proteins control a wide variety of processes during tissue development and homeostasis. However, their role in regulation of stem cell properties during tooth development remains elusive. Here, we revealed that dental pulp stem cells (DPSCs) express IFT80, which is required for maintaining DPSC properties. Mice with deletion of IFT80 in odontoblast lineage show impaired molar root development and delayed incisor eruption through reduced DPSC proliferation and differentiation, and disrupted odontoblast polarization. Impaired odontoblast differentiation resulted from disrupted hedgehog (Hh) signaling pathways. Decreased DPSC proliferation is associated with impaired fibroblast growth factor 2 (FGF2) signaling caused by loss of IFT80, leading to the disruption of FGF2-FGFR1-PI3K-AKT signaling in IFT80-deficient DPSCs. The results provide the first evidence that IFT80 controls tooth development through influencing cell proliferation, differentiation, and polarization, and Hh and FGF/AKT signaling pathways, demonstrating that IFT proteins are likely to be the new therapeutic targets for tooth and other tissue repair and regeneration.
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spelling pubmed-63476322019-01-28 IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development Yuan, Xue Cao, Xu Yang, Shuying Cell Death Dis Article Primary cilia and intraflagellar transport (IFT) proteins control a wide variety of processes during tissue development and homeostasis. However, their role in regulation of stem cell properties during tooth development remains elusive. Here, we revealed that dental pulp stem cells (DPSCs) express IFT80, which is required for maintaining DPSC properties. Mice with deletion of IFT80 in odontoblast lineage show impaired molar root development and delayed incisor eruption through reduced DPSC proliferation and differentiation, and disrupted odontoblast polarization. Impaired odontoblast differentiation resulted from disrupted hedgehog (Hh) signaling pathways. Decreased DPSC proliferation is associated with impaired fibroblast growth factor 2 (FGF2) signaling caused by loss of IFT80, leading to the disruption of FGF2-FGFR1-PI3K-AKT signaling in IFT80-deficient DPSCs. The results provide the first evidence that IFT80 controls tooth development through influencing cell proliferation, differentiation, and polarization, and Hh and FGF/AKT signaling pathways, demonstrating that IFT proteins are likely to be the new therapeutic targets for tooth and other tissue repair and regeneration. Nature Publishing Group UK 2019-01-25 /pmc/articles/PMC6347632/ /pubmed/30683845 http://dx.doi.org/10.1038/s41419-018-0951-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yuan, Xue
Cao, Xu
Yang, Shuying
IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title_full IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title_fullStr IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title_full_unstemmed IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title_short IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
title_sort ift80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347632/
https://www.ncbi.nlm.nih.gov/pubmed/30683845
http://dx.doi.org/10.1038/s41419-018-0951-9
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