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Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite

BACKGROUND: Polyetheretherketone (PEEK) exhibits stable chemical properties, excellent biocompatibility, and rational mechanical properties that are similar to those of human cortical bone, but the lack of bioactivity impedes its clinical application. METHODS: In this study, hydroxyapatite (HA) was...

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Autores principales: Ma, Rui, Guo, Dagang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347847/
https://www.ncbi.nlm.nih.gov/pubmed/30683125
http://dx.doi.org/10.1186/s13018-019-1069-1
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author Ma, Rui
Guo, Dagang
author_facet Ma, Rui
Guo, Dagang
author_sort Ma, Rui
collection PubMed
description BACKGROUND: Polyetheretherketone (PEEK) exhibits stable chemical properties, excellent biocompatibility, and rational mechanical properties that are similar to those of human cortical bone, but the lack of bioactivity impedes its clinical application. METHODS: In this study, hydroxyapatite (HA) was incorporated into PEEK to fabricate HA/PEEK biocomposite using a compounding and injection-molding technique. The tensile properties of the prepared HA/PEEK composites (HA content from 0 to 40 wt%) were tested to choose an optimal HA content. To evaluate the bioactivity of the composite, the cell attachment, proliferation, spreading and alkaline phosphatase (ALP) activity of MC3T3-E1 cells, and apatite formation after immersion in simulated body fluid (SBF), and osseointegration in a rabbit cranial defect model were investigated. The results were compared to those from ultra-high molecular weight polyethylene (UHMWPE) and pure PEEK. RESULTS: By evaluating the tensile properties and elastic moduli of PEEK composite samples/PEEK composites with different HA contents, the 30 wt% HA/PEEK composite was chosen for use in the subsequent tests. The results of the cell tests demonstrated that PEEK composite samples/PEEK composite exhibited better cell attachment, proliferation, spreading, and higher ALP activity than those of UHMWPE and pure PEEK. Apatite islands formed on the HA/PEEK composite after immersion in SBF for 7 days and grew continuously with longer time periods. Animal tests indicated that bone contact and new bone formation around the HA/PEEK composite were more obvious than those around UHMWPE and pure PEEK. CONCLUSIONS: The HA/PEEK biocomposite created by a compounding and injection-molding technique exhibited enhanced osteogenesis and could be used as a candidate of orthopedic implants.
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spelling pubmed-63478472019-01-30 Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite Ma, Rui Guo, Dagang J Orthop Surg Res Research Article BACKGROUND: Polyetheretherketone (PEEK) exhibits stable chemical properties, excellent biocompatibility, and rational mechanical properties that are similar to those of human cortical bone, but the lack of bioactivity impedes its clinical application. METHODS: In this study, hydroxyapatite (HA) was incorporated into PEEK to fabricate HA/PEEK biocomposite using a compounding and injection-molding technique. The tensile properties of the prepared HA/PEEK composites (HA content from 0 to 40 wt%) were tested to choose an optimal HA content. To evaluate the bioactivity of the composite, the cell attachment, proliferation, spreading and alkaline phosphatase (ALP) activity of MC3T3-E1 cells, and apatite formation after immersion in simulated body fluid (SBF), and osseointegration in a rabbit cranial defect model were investigated. The results were compared to those from ultra-high molecular weight polyethylene (UHMWPE) and pure PEEK. RESULTS: By evaluating the tensile properties and elastic moduli of PEEK composite samples/PEEK composites with different HA contents, the 30 wt% HA/PEEK composite was chosen for use in the subsequent tests. The results of the cell tests demonstrated that PEEK composite samples/PEEK composite exhibited better cell attachment, proliferation, spreading, and higher ALP activity than those of UHMWPE and pure PEEK. Apatite islands formed on the HA/PEEK composite after immersion in SBF for 7 days and grew continuously with longer time periods. Animal tests indicated that bone contact and new bone formation around the HA/PEEK composite were more obvious than those around UHMWPE and pure PEEK. CONCLUSIONS: The HA/PEEK biocomposite created by a compounding and injection-molding technique exhibited enhanced osteogenesis and could be used as a candidate of orthopedic implants. BioMed Central 2019-01-25 /pmc/articles/PMC6347847/ /pubmed/30683125 http://dx.doi.org/10.1186/s13018-019-1069-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ma, Rui
Guo, Dagang
Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title_full Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title_fullStr Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title_full_unstemmed Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title_short Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
title_sort evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347847/
https://www.ncbi.nlm.nih.gov/pubmed/30683125
http://dx.doi.org/10.1186/s13018-019-1069-1
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