A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level

BACKGROUND: To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of...

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Autores principales: Wu, Yu, Wang, Miaomiao, Zhang, Jinlong, Sun, Na, Li, Chunping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348078/
https://www.ncbi.nlm.nih.gov/pubmed/30700972
http://dx.doi.org/10.1186/s41065-019-0084-x
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author Wu, Yu
Wang, Miaomiao
Zhang, Jinlong
Sun, Na
Li, Chunping
author_facet Wu, Yu
Wang, Miaomiao
Zhang, Jinlong
Sun, Na
Li, Chunping
author_sort Wu, Yu
collection PubMed
description BACKGROUND: To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of lead poisoning. RESULTS: Serum AGT levels were significantly higher in individuals carrying the rs7079 CA genotype, as compared to those carrying the rs7079 CC genotype. The binding of the miRNA mimics miR-31-5p and miR-584-5p to the 3′ UTR of AGT differed based on which rs7079 variant was present, implying that AGT gene expression depends on the rs7079 variant carried. CONCLUSIONS: The rs7079 C to A substitution reduced the binding of miR-31-5p/miR-584-5p to the 3′ UTR of AGT, possibly altering the risk of lead poisoning.
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spelling pubmed-63480782019-01-30 A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level Wu, Yu Wang, Miaomiao Zhang, Jinlong Sun, Na Li, Chunping Hereditas Research BACKGROUND: To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of lead poisoning. RESULTS: Serum AGT levels were significantly higher in individuals carrying the rs7079 CA genotype, as compared to those carrying the rs7079 CC genotype. The binding of the miRNA mimics miR-31-5p and miR-584-5p to the 3′ UTR of AGT differed based on which rs7079 variant was present, implying that AGT gene expression depends on the rs7079 variant carried. CONCLUSIONS: The rs7079 C to A substitution reduced the binding of miR-31-5p/miR-584-5p to the 3′ UTR of AGT, possibly altering the risk of lead poisoning. BioMed Central 2019-01-26 /pmc/articles/PMC6348078/ /pubmed/30700972 http://dx.doi.org/10.1186/s41065-019-0084-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Yu
Wang, Miaomiao
Zhang, Jinlong
Sun, Na
Li, Chunping
A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title_full A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title_fullStr A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title_full_unstemmed A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title_short A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
title_sort new model of the mechanism underlying lead poisoning: snp in mirna target region influence the agt expression level
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348078/
https://www.ncbi.nlm.nih.gov/pubmed/30700972
http://dx.doi.org/10.1186/s41065-019-0084-x
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