Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-processed passenger strand. Recent studies have shown that shortening the stem of conventional shRNAs abolishes passenger strand release. These Dicer-independent shRNAs, expressed from RNA polymerase III (Pol I...

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Detalles Bibliográficos
Autores principales: Kaadt, Erik, Alsing, Sidsel, Cecchi, Claudia R., Damgaard, Christian Kroun, Corydon, Thomas J., Aagaard, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348697/
https://www.ncbi.nlm.nih.gov/pubmed/30654192
http://dx.doi.org/10.1016/j.omtn.2018.11.013

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