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Yersinia Pseudotuberculosis Modulates Regulatory T Cell Stability via Injection of Yersinia Outer Proteins in a Type III Secretion System-Dependent Manner

Adaptive immunity is essentially required to control acute infection with enteropathogenic Yersinia pseudotuberculosis (Yptb). We have recently demonstrated that Yptb can directly modulate naïve CD4(+) T cell differentiation. However, whether fully differentiated forkhead box protein P3 (Foxp3(+)) r...

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Detalles Bibliográficos
Autores principales: Elfiky, Ahmed, Bonifacius, Agnes, Pezoldt, Joern, Pasztoi, Maria, Chaoprasid, Paweena, Sadana, Pooja, El-Sherbeeny, Nagla, Hagras, Magda, Scrima, Andrea, Dersch, Petra, Huehn, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Akadémiai Kiadó 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348704/
https://www.ncbi.nlm.nih.gov/pubmed/30719325
http://dx.doi.org/10.1556/1886.2018.00015
Descripción
Sumario:Adaptive immunity is essentially required to control acute infection with enteropathogenic Yersinia pseudotuberculosis (Yptb). We have recently demonstrated that Yptb can directly modulate naïve CD4(+) T cell differentiation. However, whether fully differentiated forkhead box protein P3 (Foxp3(+)) regulatory T cells (Tregs), fundamental key players to maintain immune homeostasis, are targeted by Yptb remains elusive. Here, we demonstrate that within the CD4(+) T cell compartment Yptb preferentially targets Tregs and injects Yersinia outer proteins (Yops) in a process that depends on the type III secretion system and invasins. Remarkably, Yop-translocation into ex vivo isolated Foxp3(+) Tregs resulted in a substantial downregulation of Foxp3 expression and a decreased capacity to express the immunosuppressive cytokine interleukin-10 (IL-10). Together, these findings highlight that invasins are critically required to mediate Yptb attachment to Foxp3(+) Tregs, which allows efficient Yop-translocation and finally enables the modulation of the Foxp3(+) Tregs' suppressive phenotype.