Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions

FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possi...

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Autores principales: Cerofolini, Linda, Giuntini, Stefano, Barbieri, Letizia, Pennestri, Matteo, Codina, Anna, Fragai, Marco, Banci, Lucia, Luchinat, Enrico, Ravera, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2019
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350048/
https://www.ncbi.nlm.nih.gov/pubmed/30580921
http://dx.doi.org/10.1016/j.bpj.2018.11.3132
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author Cerofolini, Linda
Giuntini, Stefano
Barbieri, Letizia
Pennestri, Matteo
Codina, Anna
Fragai, Marco
Banci, Lucia
Luchinat, Enrico
Ravera, Enrico
author_facet Cerofolini, Linda
Giuntini, Stefano
Barbieri, Letizia
Pennestri, Matteo
Codina, Anna
Fragai, Marco
Banci, Lucia
Luchinat, Enrico
Ravera, Enrico
author_sort Cerofolini, Linda
collection PubMed
description FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies.
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spelling pubmed-63500482020-01-22 Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions Cerofolini, Linda Giuntini, Stefano Barbieri, Letizia Pennestri, Matteo Codina, Anna Fragai, Marco Banci, Lucia Luchinat, Enrico Ravera, Enrico Biophys J Articles FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies. The Biophysical Society 2019-01-22 2018-12-06 /pmc/articles/PMC6350048/ /pubmed/30580921 http://dx.doi.org/10.1016/j.bpj.2018.11.3132 Text en © 2018 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles
Cerofolini, Linda
Giuntini, Stefano
Barbieri, Letizia
Pennestri, Matteo
Codina, Anna
Fragai, Marco
Banci, Lucia
Luchinat, Enrico
Ravera, Enrico
Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title_full Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title_fullStr Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title_full_unstemmed Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title_short Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
title_sort real-time insights into biological events: in-cell processes and protein-ligand interactions
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350048/
https://www.ncbi.nlm.nih.gov/pubmed/30580921
http://dx.doi.org/10.1016/j.bpj.2018.11.3132
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