Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model

BACKGROUND: Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resve...

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Autores principales: Zhang, Guiqi, Liu, Yi, Xu, Lichen, Sha, Chunhe, Zhang, Haibin, Xu, Weibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350285/
https://www.ncbi.nlm.nih.gov/pubmed/30691440
http://dx.doi.org/10.1186/s12896-019-0502-1
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author Zhang, Guiqi
Liu, Yi
Xu, Lichen
Sha, Chunhe
Zhang, Haibin
Xu, Weibing
author_facet Zhang, Guiqi
Liu, Yi
Xu, Lichen
Sha, Chunhe
Zhang, Haibin
Xu, Weibing
author_sort Zhang, Guiqi
collection PubMed
description BACKGROUND: Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS. RESULTS: RSV improved inflammatory injury and functional recovery in rat model of SCI. RSV inhibited LPS-induced inflammatory injury in PC-12 cells via increasing viability, decreasing apoptosis, and suppressing IL-1β, IL-6, and TNF-α expression. miR-132 was down-regulated after LPS treatment but up-regulated after RSV administration. miR-132 silence curbed the protective effect of RSV. The results including increase of cell growth, suppression of inflammatory response, and blocking of NF-κB and p38MAPK pathways produced by RSV were all reversed by miR-132 silence. CONCLUSION: RSV could up-regulate miR-132 and further ameliorate inflammatory response in PC-12 cells by inhibiting NF-κB and p38MAPK pathways.
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spelling pubmed-63502852019-02-04 Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model Zhang, Guiqi Liu, Yi Xu, Lichen Sha, Chunhe Zhang, Haibin Xu, Weibing BMC Biotechnol Research Article BACKGROUND: Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS. RESULTS: RSV improved inflammatory injury and functional recovery in rat model of SCI. RSV inhibited LPS-induced inflammatory injury in PC-12 cells via increasing viability, decreasing apoptosis, and suppressing IL-1β, IL-6, and TNF-α expression. miR-132 was down-regulated after LPS treatment but up-regulated after RSV administration. miR-132 silence curbed the protective effect of RSV. The results including increase of cell growth, suppression of inflammatory response, and blocking of NF-κB and p38MAPK pathways produced by RSV were all reversed by miR-132 silence. CONCLUSION: RSV could up-regulate miR-132 and further ameliorate inflammatory response in PC-12 cells by inhibiting NF-κB and p38MAPK pathways. BioMed Central 2019-01-28 /pmc/articles/PMC6350285/ /pubmed/30691440 http://dx.doi.org/10.1186/s12896-019-0502-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Guiqi
Liu, Yi
Xu, Lichen
Sha, Chunhe
Zhang, Haibin
Xu, Weibing
Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title_full Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title_fullStr Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title_full_unstemmed Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title_short Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model
title_sort resveratrol alleviates lipopolysaccharide-induced inflammation in pc-12 cells and in rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350285/
https://www.ncbi.nlm.nih.gov/pubmed/30691440
http://dx.doi.org/10.1186/s12896-019-0502-1
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