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Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells

BACKGROUND: Olanzapine (OLZ) is a second-generation antipsychotic drug used for treatment of schizophrenia, bipolar disorder, and other neuropsychiatric conditions. Undesirable side effects of OLZ include metabolic alterations associated with chronic oxidative-inflammation events. It is possible tha...

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Autores principales: Fernandes, Marcelo Soares, Barbisan, Fernanda, Azzolin, Verônica Farina, do Prado-Lima, Pedro Antônio Schmidt, Teixeira, Cibele Ferreira, da Cruz Jung, Ivo Emílio, Assmann, Charles Elias, Riffel, Rogerio Tomasi, Duarte, Marta Maria Medeiros Frescura, Aguiar- Ribeiro, Ednea Maia, da Cruz, Ivana Beatrice Mânica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350970/
https://www.ncbi.nlm.nih.gov/pubmed/30695037
http://dx.doi.org/10.1371/journal.pone.0209223
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author Fernandes, Marcelo Soares
Barbisan, Fernanda
Azzolin, Verônica Farina
do Prado-Lima, Pedro Antônio Schmidt
Teixeira, Cibele Ferreira
da Cruz Jung, Ivo Emílio
Assmann, Charles Elias
Riffel, Rogerio Tomasi
Duarte, Marta Maria Medeiros Frescura
Aguiar- Ribeiro, Ednea Maia
da Cruz, Ivana Beatrice Mânica
author_facet Fernandes, Marcelo Soares
Barbisan, Fernanda
Azzolin, Verônica Farina
do Prado-Lima, Pedro Antônio Schmidt
Teixeira, Cibele Ferreira
da Cruz Jung, Ivo Emílio
Assmann, Charles Elias
Riffel, Rogerio Tomasi
Duarte, Marta Maria Medeiros Frescura
Aguiar- Ribeiro, Ednea Maia
da Cruz, Ivana Beatrice Mânica
author_sort Fernandes, Marcelo Soares
collection PubMed
description BACKGROUND: Olanzapine (OLZ) is a second-generation antipsychotic drug used for treatment of schizophrenia, bipolar disorder, and other neuropsychiatric conditions. Undesirable side effects of OLZ include metabolic alterations associated with chronic oxidative-inflammation events. It is possible that lithium (Li), a mood modulator that exhibits anti-inflammatory properties may attenuate OLZ-induced oxi-inflammatory effects. METHODOLOGY: To test this hypothesis we activated RAW 264.7 immortalized macrophages with OLZ and evaluated oxidation and inflammation at the gene and protein levels. Li and OLZ concentrations were determined using estimated plasma therapeutic concentrations. RESULTS: OLZ triggered a significant increase in macrophage proliferation at 72 h. Higher levels of oxidative markers and proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, with a concomitant reduction in IL-10, were observed in OLZ-exposed macrophages. Lithium (Li) exposure triggered a short and attenuated inflammatory response demonstrated by elevation of superoxide anion (SA), reactive oxygen species (ROS), IL-1β, and cellular proliferation followed by elevation of anti-inflammatory IL-10 levels. Li treatment of OLZ-supplemented macrophages was able to reverse elevation of oxidative and inflammatory markers and increase IL-10 levels. CONCLUSIONS: Despite methodological limitations related to in vitro protocols, results suggested that Li may attenuate OLZ-induced oxidative and inflammatory responses that result from metabolic side effects associated with OLZ.
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spelling pubmed-63509702019-02-15 Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells Fernandes, Marcelo Soares Barbisan, Fernanda Azzolin, Verônica Farina do Prado-Lima, Pedro Antônio Schmidt Teixeira, Cibele Ferreira da Cruz Jung, Ivo Emílio Assmann, Charles Elias Riffel, Rogerio Tomasi Duarte, Marta Maria Medeiros Frescura Aguiar- Ribeiro, Ednea Maia da Cruz, Ivana Beatrice Mânica PLoS One Research Article BACKGROUND: Olanzapine (OLZ) is a second-generation antipsychotic drug used for treatment of schizophrenia, bipolar disorder, and other neuropsychiatric conditions. Undesirable side effects of OLZ include metabolic alterations associated with chronic oxidative-inflammation events. It is possible that lithium (Li), a mood modulator that exhibits anti-inflammatory properties may attenuate OLZ-induced oxi-inflammatory effects. METHODOLOGY: To test this hypothesis we activated RAW 264.7 immortalized macrophages with OLZ and evaluated oxidation and inflammation at the gene and protein levels. Li and OLZ concentrations were determined using estimated plasma therapeutic concentrations. RESULTS: OLZ triggered a significant increase in macrophage proliferation at 72 h. Higher levels of oxidative markers and proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, with a concomitant reduction in IL-10, were observed in OLZ-exposed macrophages. Lithium (Li) exposure triggered a short and attenuated inflammatory response demonstrated by elevation of superoxide anion (SA), reactive oxygen species (ROS), IL-1β, and cellular proliferation followed by elevation of anti-inflammatory IL-10 levels. Li treatment of OLZ-supplemented macrophages was able to reverse elevation of oxidative and inflammatory markers and increase IL-10 levels. CONCLUSIONS: Despite methodological limitations related to in vitro protocols, results suggested that Li may attenuate OLZ-induced oxidative and inflammatory responses that result from metabolic side effects associated with OLZ. Public Library of Science 2019-01-29 /pmc/articles/PMC6350970/ /pubmed/30695037 http://dx.doi.org/10.1371/journal.pone.0209223 Text en © 2019 Fernandes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fernandes, Marcelo Soares
Barbisan, Fernanda
Azzolin, Verônica Farina
do Prado-Lima, Pedro Antônio Schmidt
Teixeira, Cibele Ferreira
da Cruz Jung, Ivo Emílio
Assmann, Charles Elias
Riffel, Rogerio Tomasi
Duarte, Marta Maria Medeiros Frescura
Aguiar- Ribeiro, Ednea Maia
da Cruz, Ivana Beatrice Mânica
Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title_full Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title_fullStr Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title_full_unstemmed Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title_short Lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
title_sort lithium is able to minimize olanzapine oxidative-inflammatory induction on macrophage cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350970/
https://www.ncbi.nlm.nih.gov/pubmed/30695037
http://dx.doi.org/10.1371/journal.pone.0209223
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