Cargando…
The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection
In the study, the effect of the TLR4 agonist Immunomax was investigated in vitro and in vivo. In particular, Immunomax was shown to polarize mouse bone marrow macrophages from the M0 and M2 states into the M1 state (ARG1 and iNOS mRNA expression levels were used to identify the mouse M1 and M2 pheno...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351037/ https://www.ncbi.nlm.nih.gov/pubmed/30713767 |
_version_ | 1783390532690509824 |
---|---|
author | Nikonova, A. A. Pichugin, A. V. Chulkina, M. M. Lebedeva, E. S. Gaisina, A. R. Shilovskiy, I. P. Ataullakhanov, R. I. Khaitov, M. R. Khaitov, R. M. |
author_facet | Nikonova, A. A. Pichugin, A. V. Chulkina, M. M. Lebedeva, E. S. Gaisina, A. R. Shilovskiy, I. P. Ataullakhanov, R. I. Khaitov, M. R. Khaitov, R. M. |
author_sort | Nikonova, A. A. |
collection | PubMed |
description | In the study, the effect of the TLR4 agonist Immunomax was investigated in vitro and in vivo. In particular, Immunomax was shown to polarize mouse bone marrow macrophages from the M0 and M2 states into the M1 state (ARG1 and iNOS mRNA expression levels were used to identify the mouse M1 and M2 phenotypes). Next, we investigated the prophylactic antiviral effect of Immunomax in both a model of mouse respiratory syncytial virus (RSV) infection and a model of RSV-induced bronchial asthma (BA) exacerbation. In the experiment with RSV-induced BA exacerbation, Immunomax-treated mice were characterized by a significant decrease of the viral load in lung homogenates, an increased amount of M1 macrophages in the lung, a tendency toward Th2-dependent ovalbumin-specific IgG1 antibodies decrease in blood serum, a significant increase in RSV-activated CD4+ T cells secreting IFNγ (Th1 cells), and a simultaneous significant decrease in the amount of CD4(+) cells secreting IL-4 (Th2 cells) in the mouse spleen, which were detected by ELISPOT 1.5 months after experiment. These findings suggest that treatment with the TLR4 agonist Immunomax polarizes the immune response towards antiviral Th1 and may be used for short-term antiviral prophylaxis to prevent acute respiratory viral infections in asthmatics. |
format | Online Article Text |
id | pubmed-6351037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-63510372019-02-01 The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection Nikonova, A. A. Pichugin, A. V. Chulkina, M. M. Lebedeva, E. S. Gaisina, A. R. Shilovskiy, I. P. Ataullakhanov, R. I. Khaitov, M. R. Khaitov, R. M. Acta Naturae Research Article In the study, the effect of the TLR4 agonist Immunomax was investigated in vitro and in vivo. In particular, Immunomax was shown to polarize mouse bone marrow macrophages from the M0 and M2 states into the M1 state (ARG1 and iNOS mRNA expression levels were used to identify the mouse M1 and M2 phenotypes). Next, we investigated the prophylactic antiviral effect of Immunomax in both a model of mouse respiratory syncytial virus (RSV) infection and a model of RSV-induced bronchial asthma (BA) exacerbation. In the experiment with RSV-induced BA exacerbation, Immunomax-treated mice were characterized by a significant decrease of the viral load in lung homogenates, an increased amount of M1 macrophages in the lung, a tendency toward Th2-dependent ovalbumin-specific IgG1 antibodies decrease in blood serum, a significant increase in RSV-activated CD4+ T cells secreting IFNγ (Th1 cells), and a simultaneous significant decrease in the amount of CD4(+) cells secreting IL-4 (Th2 cells) in the mouse spleen, which were detected by ELISPOT 1.5 months after experiment. These findings suggest that treatment with the TLR4 agonist Immunomax polarizes the immune response towards antiviral Th1 and may be used for short-term antiviral prophylaxis to prevent acute respiratory viral infections in asthmatics. A.I. Gordeyev 2018 /pmc/articles/PMC6351037/ /pubmed/30713767 Text en Copyright ® 2018 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nikonova, A. A. Pichugin, A. V. Chulkina, M. M. Lebedeva, E. S. Gaisina, A. R. Shilovskiy, I. P. Ataullakhanov, R. I. Khaitov, M. R. Khaitov, R. M. The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title | The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title_full | The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title_fullStr | The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title_full_unstemmed | The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title_short | The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection |
title_sort | tlr4 agonist immunomax affects the phenotype of mouse lung macrophages during respiratory syncytial virus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351037/ https://www.ncbi.nlm.nih.gov/pubmed/30713767 |
work_keys_str_mv | AT nikonovaaa thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT pichuginav thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT chulkinamm thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT lebedevaes thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT gaisinaar thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT shilovskiyip thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT ataullakhanovri thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT khaitovmr thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT khaitovrm thetlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT nikonovaaa tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT pichuginav tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT chulkinamm tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT lebedevaes tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT gaisinaar tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT shilovskiyip tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT ataullakhanovri tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT khaitovmr tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection AT khaitovrm tlr4agonistimmunomaxaffectsthephenotypeofmouselungmacrophagesduringrespiratorysyncytialvirusinfection |