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The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain geno...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351104/ https://www.ncbi.nlm.nih.gov/pubmed/30575518 http://dx.doi.org/10.7554/eLife.35478 |
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author | Davis, Gregory M Tu, Shikui Anderson, Joshua WT Colson, Rhys N Gunzburg, Menachem J Francisco, Michelle A Ray, Debashish Shrubsole, Sean P Sobotka, Julia A Seroussi, Uri Lao, Robert X Maity, Tuhin Wu, Monica Z McJunkin, Katherine Morris, Quaid D Hughes, Timothy R Wilce, Jacqueline A Claycomb, Julie M Weng, Zhiping Boag, Peter R |
author_facet | Davis, Gregory M Tu, Shikui Anderson, Joshua WT Colson, Rhys N Gunzburg, Menachem J Francisco, Michelle A Ray, Debashish Shrubsole, Sean P Sobotka, Julia A Seroussi, Uri Lao, Robert X Maity, Tuhin Wu, Monica Z McJunkin, Katherine Morris, Quaid D Hughes, Timothy R Wilce, Jacqueline A Claycomb, Julie M Weng, Zhiping Boag, Peter R |
author_sort | Davis, Gregory M |
collection | PubMed |
description | Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma. |
format | Online Article Text |
id | pubmed-6351104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63511042019-01-30 The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans Davis, Gregory M Tu, Shikui Anderson, Joshua WT Colson, Rhys N Gunzburg, Menachem J Francisco, Michelle A Ray, Debashish Shrubsole, Sean P Sobotka, Julia A Seroussi, Uri Lao, Robert X Maity, Tuhin Wu, Monica Z McJunkin, Katherine Morris, Quaid D Hughes, Timothy R Wilce, Jacqueline A Claycomb, Julie M Weng, Zhiping Boag, Peter R eLife Chromosomes and Gene Expression Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma. eLife Sciences Publications, Ltd 2018-12-21 /pmc/articles/PMC6351104/ /pubmed/30575518 http://dx.doi.org/10.7554/eLife.35478 Text en http://creativecommons.org/publicdomain/zero/1.0/ http://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (http://creativecommons.org/publicdomain/zero/1.0/) . |
spellingShingle | Chromosomes and Gene Expression Davis, Gregory M Tu, Shikui Anderson, Joshua WT Colson, Rhys N Gunzburg, Menachem J Francisco, Michelle A Ray, Debashish Shrubsole, Sean P Sobotka, Julia A Seroussi, Uri Lao, Robert X Maity, Tuhin Wu, Monica Z McJunkin, Katherine Morris, Quaid D Hughes, Timothy R Wilce, Jacqueline A Claycomb, Julie M Weng, Zhiping Boag, Peter R The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title | The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title_full | The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title_fullStr | The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title_full_unstemmed | The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title_short | The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans |
title_sort | trim-nhl protein nhl-2 is a co-factor in the nuclear and somatic rnai pathways in c. elegans |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351104/ https://www.ncbi.nlm.nih.gov/pubmed/30575518 http://dx.doi.org/10.7554/eLife.35478 |
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