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Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure

Glufosinate is a common herbicide with neurotoxic effects, leading to seizures, convulsions and memory loss. Glufosinate indirectly induces glutamate toxicity by inhibiting glutamine synthesis in astrocytes. Here, we studied the acute toxic effects of a glufosinate-based herbicide in rat optic nerve...

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Autores principales: Çomaklı, Selim, Sevim, Çiğdem, Kontadakis, George, Doğan, Elif, Taghizadehghalehjoughi, Ali, Özkaraca, Mustafa, Aschner, Michael, Nikolouzakis, Taxiarchis Konstantinos, Tsatsakis, Aristides
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351388/
https://www.ncbi.nlm.nih.gov/pubmed/30723690
http://dx.doi.org/10.1016/j.toxrep.2019.01.004
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author Çomaklı, Selim
Sevim, Çiğdem
Kontadakis, George
Doğan, Elif
Taghizadehghalehjoughi, Ali
Özkaraca, Mustafa
Aschner, Michael
Nikolouzakis, Taxiarchis Konstantinos
Tsatsakis, Aristides
author_facet Çomaklı, Selim
Sevim, Çiğdem
Kontadakis, George
Doğan, Elif
Taghizadehghalehjoughi, Ali
Özkaraca, Mustafa
Aschner, Michael
Nikolouzakis, Taxiarchis Konstantinos
Tsatsakis, Aristides
author_sort Çomaklı, Selim
collection PubMed
description Glufosinate is a common herbicide with neurotoxic effects, leading to seizures, convulsions and memory loss. Glufosinate indirectly induces glutamate toxicity by inhibiting glutamine synthesis in astrocytes. Here, we studied the acute toxic effects of a glufosinate-based herbicide in rat optic nerve at three doses (40, 80 or 120 μM, equal to 714 or 21 mg/kg bw/day). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, glucose, calcium, as well as creatinine concentrations were analyzed after 24, 48 and 72 h treatment. Intraocular pressure (IOP) (expressed as the average of both eyes) was measured with a rebound tonometer. Interleukin-1β (IL-1β) and c-Fos expression were determined by immunohistochemistry. The results established that the glufosinate-based herbicide significantly increased IL-1β and c-Fos immunopositivity in the optic nerve (p < 0.05), concomitant with increased IOP. These results suggest that commercial formulations of glufosinate acutely affect the optic nerve.
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spelling pubmed-63513882019-02-05 Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure Çomaklı, Selim Sevim, Çiğdem Kontadakis, George Doğan, Elif Taghizadehghalehjoughi, Ali Özkaraca, Mustafa Aschner, Michael Nikolouzakis, Taxiarchis Konstantinos Tsatsakis, Aristides Toxicol Rep Article Glufosinate is a common herbicide with neurotoxic effects, leading to seizures, convulsions and memory loss. Glufosinate indirectly induces glutamate toxicity by inhibiting glutamine synthesis in astrocytes. Here, we studied the acute toxic effects of a glufosinate-based herbicide in rat optic nerve at three doses (40, 80 or 120 μM, equal to 714 or 21 mg/kg bw/day). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, glucose, calcium, as well as creatinine concentrations were analyzed after 24, 48 and 72 h treatment. Intraocular pressure (IOP) (expressed as the average of both eyes) was measured with a rebound tonometer. Interleukin-1β (IL-1β) and c-Fos expression were determined by immunohistochemistry. The results established that the glufosinate-based herbicide significantly increased IL-1β and c-Fos immunopositivity in the optic nerve (p < 0.05), concomitant with increased IOP. These results suggest that commercial formulations of glufosinate acutely affect the optic nerve. Elsevier 2019-01-16 /pmc/articles/PMC6351388/ /pubmed/30723690 http://dx.doi.org/10.1016/j.toxrep.2019.01.004 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Çomaklı, Selim
Sevim, Çiğdem
Kontadakis, George
Doğan, Elif
Taghizadehghalehjoughi, Ali
Özkaraca, Mustafa
Aschner, Michael
Nikolouzakis, Taxiarchis Konstantinos
Tsatsakis, Aristides
Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title_full Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title_fullStr Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title_full_unstemmed Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title_short Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
title_sort acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-fos protein levels, as well as intraocular pressure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351388/
https://www.ncbi.nlm.nih.gov/pubmed/30723690
http://dx.doi.org/10.1016/j.toxrep.2019.01.004
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