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Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation

Mammalian hibernation is a seasonal phenomenon. The hibernation season consists of torpor periods with a reduced body temperature (Tb), interrupted by euthermic arousal periods (interbout arousal, IBA). The physiological changes associated with hibernation are assumed to be under genetic control. Ho...

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Autores principales: Tsukamoto, Daisuke, Hasegawa, Tomoko, Hirose, Shin-ichi, Sakurai, Yukina, Ito, Michihiko, Takamatsu, Nobuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351659/
https://www.ncbi.nlm.nih.gov/pubmed/30696859
http://dx.doi.org/10.1038/s41598-018-37022-7
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author Tsukamoto, Daisuke
Hasegawa, Tomoko
Hirose, Shin-ichi
Sakurai, Yukina
Ito, Michihiko
Takamatsu, Nobuhiko
author_facet Tsukamoto, Daisuke
Hasegawa, Tomoko
Hirose, Shin-ichi
Sakurai, Yukina
Ito, Michihiko
Takamatsu, Nobuhiko
author_sort Tsukamoto, Daisuke
collection PubMed
description Mammalian hibernation is a seasonal phenomenon. The hibernation season consists of torpor periods with a reduced body temperature (Tb), interrupted by euthermic arousal periods (interbout arousal, IBA). The physiological changes associated with hibernation are assumed to be under genetic control. However, the molecular mechanisms that govern hibernation-associated gene regulation are still unclear. We found that HSP70 transcription is upregulated in the liver of nonhibernating (summer-active) chipmunks compared with hibernating (winter-torpid) ones. In parallel, HSF1, the major transcription factor for HSP70 expression, is abundant in the liver-cell nuclei of nonhibernating chipmunks, and disappears from the nuclei of hibernating ones. Moreover, during IBA, HSF1 reappears in the nuclei and drives HSP70 transcription. In mouse liver, HSF1 is regulated by the daily Tb rhythm, and acts as a circadian transcription factor. Taken together, chipmunks similarly use the Tb rhythm to regulate gene expression via HSF1 during the torpor-arousal cycle in the hibernation season.
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spelling pubmed-63516592019-01-31 Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation Tsukamoto, Daisuke Hasegawa, Tomoko Hirose, Shin-ichi Sakurai, Yukina Ito, Michihiko Takamatsu, Nobuhiko Sci Rep Article Mammalian hibernation is a seasonal phenomenon. The hibernation season consists of torpor periods with a reduced body temperature (Tb), interrupted by euthermic arousal periods (interbout arousal, IBA). The physiological changes associated with hibernation are assumed to be under genetic control. However, the molecular mechanisms that govern hibernation-associated gene regulation are still unclear. We found that HSP70 transcription is upregulated in the liver of nonhibernating (summer-active) chipmunks compared with hibernating (winter-torpid) ones. In parallel, HSF1, the major transcription factor for HSP70 expression, is abundant in the liver-cell nuclei of nonhibernating chipmunks, and disappears from the nuclei of hibernating ones. Moreover, during IBA, HSF1 reappears in the nuclei and drives HSP70 transcription. In mouse liver, HSF1 is regulated by the daily Tb rhythm, and acts as a circadian transcription factor. Taken together, chipmunks similarly use the Tb rhythm to regulate gene expression via HSF1 during the torpor-arousal cycle in the hibernation season. Nature Publishing Group UK 2019-01-29 /pmc/articles/PMC6351659/ /pubmed/30696859 http://dx.doi.org/10.1038/s41598-018-37022-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tsukamoto, Daisuke
Hasegawa, Tomoko
Hirose, Shin-ichi
Sakurai, Yukina
Ito, Michihiko
Takamatsu, Nobuhiko
Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title_full Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title_fullStr Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title_full_unstemmed Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title_short Circadian transcription factor HSF1 regulates differential HSP70 gene transcription during the arousal-torpor cycle in mammalian hibernation
title_sort circadian transcription factor hsf1 regulates differential hsp70 gene transcription during the arousal-torpor cycle in mammalian hibernation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351659/
https://www.ncbi.nlm.nih.gov/pubmed/30696859
http://dx.doi.org/10.1038/s41598-018-37022-7
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