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Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351762/ https://www.ncbi.nlm.nih.gov/pubmed/30481988 http://dx.doi.org/10.4142/jvs.2019.20.1.79 |
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author | Abdillah, Dimas A. Setyawan, Erif M. N. Oh, Hyun Ju Ra, Kihae Lee, Seok Hee Kim, Min Jung Lee, Byeong Chun |
author_facet | Abdillah, Dimas A. Setyawan, Erif M. N. Oh, Hyun Ju Ra, Kihae Lee, Seok Hee Kim, Min Jung Lee, Byeong Chun |
author_sort | Abdillah, Dimas A. |
collection | PubMed |
description | The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa. |
format | Online Article Text |
id | pubmed-6351762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63517622019-02-08 Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm Abdillah, Dimas A. Setyawan, Erif M. N. Oh, Hyun Ju Ra, Kihae Lee, Seok Hee Kim, Min Jung Lee, Byeong Chun J Vet Sci Original Article The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa. The Korean Society of Veterinary Science 2019-01 2019-01-24 /pmc/articles/PMC6351762/ /pubmed/30481988 http://dx.doi.org/10.4142/jvs.2019.20.1.79 Text en © 2019 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Abdillah, Dimas A. Setyawan, Erif M. N. Oh, Hyun Ju Ra, Kihae Lee, Seok Hee Kim, Min Jung Lee, Byeong Chun Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title | Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title_full | Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title_fullStr | Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title_full_unstemmed | Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title_short | Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
title_sort | iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351762/ https://www.ncbi.nlm.nih.gov/pubmed/30481988 http://dx.doi.org/10.4142/jvs.2019.20.1.79 |
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