Genetic identification of astroviruses in wild boars

Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Ove...

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Autores principales: Vilcek, Stefan, Salamunova, Slavomira, Jackova, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2019
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351768/
https://www.ncbi.nlm.nih.gov/pubmed/30481990
http://dx.doi.org/10.4142/jvs.2019.20.1.91
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author Vilcek, Stefan
Salamunova, Slavomira
Jackova, Anna
author_facet Vilcek, Stefan
Salamunova, Slavomira
Jackova, Anna
author_sort Vilcek, Stefan
collection PubMed
description Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
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spelling pubmed-63517682019-02-08 Genetic identification of astroviruses in wild boars Vilcek, Stefan Salamunova, Slavomira Jackova, Anna J Vet Sci Short Communication Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission. The Korean Society of Veterinary Science 2019-01 2019-01-24 /pmc/articles/PMC6351768/ /pubmed/30481990 http://dx.doi.org/10.4142/jvs.2019.20.1.91 Text en © 2019 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Vilcek, Stefan
Salamunova, Slavomira
Jackova, Anna
Genetic identification of astroviruses in wild boars
title Genetic identification of astroviruses in wild boars
title_full Genetic identification of astroviruses in wild boars
title_fullStr Genetic identification of astroviruses in wild boars
title_full_unstemmed Genetic identification of astroviruses in wild boars
title_short Genetic identification of astroviruses in wild boars
title_sort genetic identification of astroviruses in wild boars
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351768/
https://www.ncbi.nlm.nih.gov/pubmed/30481990
http://dx.doi.org/10.4142/jvs.2019.20.1.91
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