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Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013
BACKGROUND: Mandatory reporting of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infections (BSI) has occurred in England for over 15years. Epidemiological information is recorded, but routine collection of isolates for characterisation has not been routinely undertaken. Ongoing dev...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351993/ https://www.ncbi.nlm.nih.gov/pubmed/30696529 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.4.1800215 |
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author | Toleman, Michelle S Reuter, Sandra Jamrozy, Dorota Wilson, Hayley J Blane, Beth Harrison, Ewan M Coll, Francesc Hope, Russell J Kearns, Angela Parkhill, Julian Peacock, Sharon J Török, M Estée |
author_facet | Toleman, Michelle S Reuter, Sandra Jamrozy, Dorota Wilson, Hayley J Blane, Beth Harrison, Ewan M Coll, Francesc Hope, Russell J Kearns, Angela Parkhill, Julian Peacock, Sharon J Török, M Estée |
author_sort | Toleman, Michelle S |
collection | PubMed |
description | BACKGROUND: Mandatory reporting of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infections (BSI) has occurred in England for over 15years. Epidemiological information is recorded, but routine collection of isolates for characterisation has not been routinely undertaken. Ongoing developments in whole-genome sequencing (WGS) have demonstrated its value in outbreak investigations and for determining the spread of antimicrobial resistance and bacterial population structure. Benefits of adding genomics to routine epidemiological MRSA surveillance are unknown. AIM: To determine feasibility and potential utility of adding genomics to epidemiological surveillance of MRSA. METHODS: We conducted an epidemiological and genomic survey of MRSA BSI in England over a 1-year period (1 October 2012–30 September 2013). RESULTS: During the study period, 903 cases of MRSA BSI were reported; 425 isolates were available for sequencing of which, 276 (65%) were clonal complex (CC) 22. Addition of 64 MRSA genomes from published outbreak investigations showed that the study genomes could provide context for outbreak isolates and supported cluster identification. Comparison to other MRSA genome collections demonstrated variation in clonal diversity achieved through different sampling strategies and identified potentially high-risk clones e.g. USA300 and local expansion of CC5 MRSA in South West England. CONCLUSIONS: We demonstrate the potential utility of combined epidemiological and genomic MRSA BSI surveillance to determine the national population structure of MRSA, contextualise previous MRSA outbreaks, and detect potentially high-risk lineages. These findings support the integration of epidemiological and genomic surveillance for MRSA BSI as a step towards a comprehensive surveillance programme in England. |
format | Online Article Text |
id | pubmed-6351993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | European Centre for Disease Prevention and Control (ECDC) |
record_format | MEDLINE/PubMed |
spelling | pubmed-63519932019-02-15 Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 Toleman, Michelle S Reuter, Sandra Jamrozy, Dorota Wilson, Hayley J Blane, Beth Harrison, Ewan M Coll, Francesc Hope, Russell J Kearns, Angela Parkhill, Julian Peacock, Sharon J Török, M Estée Euro Surveill Research BACKGROUND: Mandatory reporting of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infections (BSI) has occurred in England for over 15years. Epidemiological information is recorded, but routine collection of isolates for characterisation has not been routinely undertaken. Ongoing developments in whole-genome sequencing (WGS) have demonstrated its value in outbreak investigations and for determining the spread of antimicrobial resistance and bacterial population structure. Benefits of adding genomics to routine epidemiological MRSA surveillance are unknown. AIM: To determine feasibility and potential utility of adding genomics to epidemiological surveillance of MRSA. METHODS: We conducted an epidemiological and genomic survey of MRSA BSI in England over a 1-year period (1 October 2012–30 September 2013). RESULTS: During the study period, 903 cases of MRSA BSI were reported; 425 isolates were available for sequencing of which, 276 (65%) were clonal complex (CC) 22. Addition of 64 MRSA genomes from published outbreak investigations showed that the study genomes could provide context for outbreak isolates and supported cluster identification. Comparison to other MRSA genome collections demonstrated variation in clonal diversity achieved through different sampling strategies and identified potentially high-risk clones e.g. USA300 and local expansion of CC5 MRSA in South West England. CONCLUSIONS: We demonstrate the potential utility of combined epidemiological and genomic MRSA BSI surveillance to determine the national population structure of MRSA, contextualise previous MRSA outbreaks, and detect potentially high-risk lineages. These findings support the integration of epidemiological and genomic surveillance for MRSA BSI as a step towards a comprehensive surveillance programme in England. European Centre for Disease Prevention and Control (ECDC) 2019-01-24 /pmc/articles/PMC6351993/ /pubmed/30696529 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.4.1800215 Text en This article is copyright of the authors or their affiliated institutions, 2019. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
spellingShingle | Research Toleman, Michelle S Reuter, Sandra Jamrozy, Dorota Wilson, Hayley J Blane, Beth Harrison, Ewan M Coll, Francesc Hope, Russell J Kearns, Angela Parkhill, Julian Peacock, Sharon J Török, M Estée Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title | Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title_full | Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title_fullStr | Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title_full_unstemmed | Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title_short | Prospective genomic surveillance of methicillin-resistant Staphylococcus aureus (MRSA) associated with bloodstream infection, England, 1 October 2012 to 30 September 2013 |
title_sort | prospective genomic surveillance of methicillin-resistant staphylococcus aureus (mrsa) associated with bloodstream infection, england, 1 october 2012 to 30 september 2013 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351993/ https://www.ncbi.nlm.nih.gov/pubmed/30696529 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.4.1800215 |
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