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Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach

Multispectral optoacoustic tomography (MSOT) represents a new imaging approach revealing functional tissue information without extrinsic contrast agents. Using a clinical combined ultrasound (US)/MSOT device, we investigated the interindividual robustness and impact of intra- and interobserver varia...

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Autores principales: Helfen, Anne, Masthoff, Max, Claussen, Jing, Gerwing, Mirjam, Heindel, Walter, Ntziachristos, Vasilis, Eisenblätter, Michel, Köhler, Michael, Wildgruber, Moritz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352009/
https://www.ncbi.nlm.nih.gov/pubmed/30634409
http://dx.doi.org/10.3390/jcm8010063
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author Helfen, Anne
Masthoff, Max
Claussen, Jing
Gerwing, Mirjam
Heindel, Walter
Ntziachristos, Vasilis
Eisenblätter, Michel
Köhler, Michael
Wildgruber, Moritz
author_facet Helfen, Anne
Masthoff, Max
Claussen, Jing
Gerwing, Mirjam
Heindel, Walter
Ntziachristos, Vasilis
Eisenblätter, Michel
Köhler, Michael
Wildgruber, Moritz
author_sort Helfen, Anne
collection PubMed
description Multispectral optoacoustic tomography (MSOT) represents a new imaging approach revealing functional tissue information without extrinsic contrast agents. Using a clinical combined ultrasound (US)/MSOT device, we investigated the interindividual robustness and impact of intra- and interobserver variability of MSOT values in soft tissue (muscle and subcutaneous fat) of healthy volunteers. Semiquantitative MSOT values for deoxygenated (Hb), oxygenated (HbO(2)) and total hemoglobin (HbT), as well as oxygen saturation (sO(2)), were calculated for both forearms in transversal and longitudinal probe orientation (n = 3, 8 measurements per subject). For intraobserver reproducibility, the same examiner investigated three subjects twice. Mean values of left vs. right forearm and transversal vs. longitudinal probe orientation were compared using an unpaired Student’s t test. Bland Altmann plots with 95% limits of agreement for absolute averages and differences were calculated. Intraclass correlation coefficients (ICC 2,k) were computed for three different examiners. We obtained reproducible and consistent MSOT values with small-to-moderate deviation for muscle and subcutaneous fat tissue. Probe orientation and body side had no impact on calculated MSOT values (p > 0.05 each). Intraobserver reproducibility revealed equable mean values with small-to-moderate deviation. For muscular tissue, good ICC was obtained for sO(2). Measurements of subcutaneous tissue revealed good-to-excellent ICCs for all calculated values. Thus, in this preliminary study on healthy individuals, clinical MSOT provided consistent and reproducible functional soft tissue characterization, independent on the investigating personnel.
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spelling pubmed-63520092019-02-01 Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach Helfen, Anne Masthoff, Max Claussen, Jing Gerwing, Mirjam Heindel, Walter Ntziachristos, Vasilis Eisenblätter, Michel Köhler, Michael Wildgruber, Moritz J Clin Med Brief Report Multispectral optoacoustic tomography (MSOT) represents a new imaging approach revealing functional tissue information without extrinsic contrast agents. Using a clinical combined ultrasound (US)/MSOT device, we investigated the interindividual robustness and impact of intra- and interobserver variability of MSOT values in soft tissue (muscle and subcutaneous fat) of healthy volunteers. Semiquantitative MSOT values for deoxygenated (Hb), oxygenated (HbO(2)) and total hemoglobin (HbT), as well as oxygen saturation (sO(2)), were calculated for both forearms in transversal and longitudinal probe orientation (n = 3, 8 measurements per subject). For intraobserver reproducibility, the same examiner investigated three subjects twice. Mean values of left vs. right forearm and transversal vs. longitudinal probe orientation were compared using an unpaired Student’s t test. Bland Altmann plots with 95% limits of agreement for absolute averages and differences were calculated. Intraclass correlation coefficients (ICC 2,k) were computed for three different examiners. We obtained reproducible and consistent MSOT values with small-to-moderate deviation for muscle and subcutaneous fat tissue. Probe orientation and body side had no impact on calculated MSOT values (p > 0.05 each). Intraobserver reproducibility revealed equable mean values with small-to-moderate deviation. For muscular tissue, good ICC was obtained for sO(2). Measurements of subcutaneous tissue revealed good-to-excellent ICCs for all calculated values. Thus, in this preliminary study on healthy individuals, clinical MSOT provided consistent and reproducible functional soft tissue characterization, independent on the investigating personnel. MDPI 2019-01-09 /pmc/articles/PMC6352009/ /pubmed/30634409 http://dx.doi.org/10.3390/jcm8010063 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Helfen, Anne
Masthoff, Max
Claussen, Jing
Gerwing, Mirjam
Heindel, Walter
Ntziachristos, Vasilis
Eisenblätter, Michel
Köhler, Michael
Wildgruber, Moritz
Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title_full Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title_fullStr Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title_full_unstemmed Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title_short Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
title_sort multispectral optoacoustic tomography: intra- and interobserver variability using a clinical hybrid approach
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352009/
https://www.ncbi.nlm.nih.gov/pubmed/30634409
http://dx.doi.org/10.3390/jcm8010063
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