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Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice

Lotus plumule is a traditional Chinese food that can be used as a beverage. In this study, three kinds of Lotus plumules from different regions of China were selected to observe the preventive effects of extracted alkaloids on CCl(4)-induced liver injuries. Animal experiments revealed that alkaloids...

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Autores principales: Liu, Bihui, Li, Ji, Yi, Ruokun, Mu, Jianfei, Zhou, Xianrong, Zhao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352077/
https://www.ncbi.nlm.nih.gov/pubmed/30669459
http://dx.doi.org/10.3390/foods8010036
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author Liu, Bihui
Li, Ji
Yi, Ruokun
Mu, Jianfei
Zhou, Xianrong
Zhao, Xin
author_facet Liu, Bihui
Li, Ji
Yi, Ruokun
Mu, Jianfei
Zhou, Xianrong
Zhao, Xin
author_sort Liu, Bihui
collection PubMed
description Lotus plumule is a traditional Chinese food that can be used as a beverage. In this study, three kinds of Lotus plumules from different regions of China were selected to observe the preventive effects of extracted alkaloids on CCl(4)-induced liver injuries. Animal experiments revealed that alkaloids extracted from Lotus plumules decreased the serum AST (aspartate aminotransferase), ALT (alanine aminotransferase), and TBIL (total bilirubin) levels, enhanced SOD (superoxide dismutase) activity, and reduced MDA (malondialdehyde) level in the liver tissues of mice with liver injury. H&E observation confirmed that alkaloids from Lotus plumules could alleviate CCl(4)-induced injuries of liver tissues and inhibit the inflammatory effect on hepatocytes. Further qPCR experiments also demonstrated that alkaloids from Lotus plumules upregulated the expression of IκB-α (inhibitor of NF-κB alpha), Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), and CAT (catalase) mRNA and downregulated TNF-α (tumor necrosis factor alpha) and NF-κB (nuclear factor kappa B) expression in the liver tissues of mice with liver injury. All three kinds of alkaloids from Lotus plumules could prevent CCl(4)-induced liver injuries by regulating the levels of oxidative stress and inflammation in mice, and the therapeutic effect was comparable to that of silymarin, the medicine commonly used in the treatment of liver diseases. In summary, alkaloids from Lotus plumules contain bioactive substances with hepatic protective efficacy and possess potential application value in the field of functional food.
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spelling pubmed-63520772019-02-01 Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice Liu, Bihui Li, Ji Yi, Ruokun Mu, Jianfei Zhou, Xianrong Zhao, Xin Foods Article Lotus plumule is a traditional Chinese food that can be used as a beverage. In this study, three kinds of Lotus plumules from different regions of China were selected to observe the preventive effects of extracted alkaloids on CCl(4)-induced liver injuries. Animal experiments revealed that alkaloids extracted from Lotus plumules decreased the serum AST (aspartate aminotransferase), ALT (alanine aminotransferase), and TBIL (total bilirubin) levels, enhanced SOD (superoxide dismutase) activity, and reduced MDA (malondialdehyde) level in the liver tissues of mice with liver injury. H&E observation confirmed that alkaloids from Lotus plumules could alleviate CCl(4)-induced injuries of liver tissues and inhibit the inflammatory effect on hepatocytes. Further qPCR experiments also demonstrated that alkaloids from Lotus plumules upregulated the expression of IκB-α (inhibitor of NF-κB alpha), Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), and CAT (catalase) mRNA and downregulated TNF-α (tumor necrosis factor alpha) and NF-κB (nuclear factor kappa B) expression in the liver tissues of mice with liver injury. All three kinds of alkaloids from Lotus plumules could prevent CCl(4)-induced liver injuries by regulating the levels of oxidative stress and inflammation in mice, and the therapeutic effect was comparable to that of silymarin, the medicine commonly used in the treatment of liver diseases. In summary, alkaloids from Lotus plumules contain bioactive substances with hepatic protective efficacy and possess potential application value in the field of functional food. MDPI 2019-01-19 /pmc/articles/PMC6352077/ /pubmed/30669459 http://dx.doi.org/10.3390/foods8010036 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Bihui
Li, Ji
Yi, Ruokun
Mu, Jianfei
Zhou, Xianrong
Zhao, Xin
Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title_full Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title_fullStr Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title_full_unstemmed Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title_short Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
title_sort preventive effect of alkaloids from lotus plumule on acute liver injury in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352077/
https://www.ncbi.nlm.nih.gov/pubmed/30669459
http://dx.doi.org/10.3390/foods8010036
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