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Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures

The aim of the study was to evaluate the effect of titanium bone fixations on mitochondrial activity, reactive oxygen species (ROS) production, glutathione metabolism, and selected markers of oxidative/nitrosative stress in the periosteum-like tissue of patients treated with mandible fractures. The...

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Autores principales: Borys, Jan, Maciejczyk, Mateusz, Antonowicz, Bożena, Krętowski, Adam, Sidun, Jarosław, Domel, Emilia, Dąbrowski, Jan Ryszard, Ładny, Jerzy Robert, Morawska, Katarzyna, Zalewska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352110/
https://www.ncbi.nlm.nih.gov/pubmed/30669680
http://dx.doi.org/10.3390/jcm8010127
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author Borys, Jan
Maciejczyk, Mateusz
Antonowicz, Bożena
Krętowski, Adam
Sidun, Jarosław
Domel, Emilia
Dąbrowski, Jan Ryszard
Ładny, Jerzy Robert
Morawska, Katarzyna
Zalewska, Anna
author_facet Borys, Jan
Maciejczyk, Mateusz
Antonowicz, Bożena
Krętowski, Adam
Sidun, Jarosław
Domel, Emilia
Dąbrowski, Jan Ryszard
Ładny, Jerzy Robert
Morawska, Katarzyna
Zalewska, Anna
author_sort Borys, Jan
collection PubMed
description The aim of the study was to evaluate the effect of titanium bone fixations on mitochondrial activity, reactive oxygen species (ROS) production, glutathione metabolism, and selected markers of oxidative/nitrosative stress in the periosteum-like tissue of patients treated with mandible fractures. The study group consisted of 30 patients with bilateral fractures of the mandible body eligible for surgical treatment. Our study is the first one that indicates disturbances of mitochondrial activity as well as a higher production of ROS in the periosteum-like tissue covering titanium fixations of the mandible. We also found significantly higher levels of reduced glutathione and enhanced activity of glutathione reductase in the periosteum homogenates of patients in the study group compared to the control group. Levels of nitrosative (S-nitrosothiols, peroxynitrite, nitrotyrosine) and oxidative stress biomarkers (malondialdehyde, protein carbonyls, dityrosine, kynurenine, and N-formylkynurenine) were statistically elevated in periosteum-like tissue covering titanium fixations. Although exposure to titanium fixations induces local antioxidant mechanisms, patients suffer oxidative damage, and in the periosteum-like tissue the phenomenon of metallosis was observed. Titanium implants cause oxidative/nitrosative stress as well as disturbances in mitochondrial activity.
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spelling pubmed-63521102019-02-01 Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures Borys, Jan Maciejczyk, Mateusz Antonowicz, Bożena Krętowski, Adam Sidun, Jarosław Domel, Emilia Dąbrowski, Jan Ryszard Ładny, Jerzy Robert Morawska, Katarzyna Zalewska, Anna J Clin Med Article The aim of the study was to evaluate the effect of titanium bone fixations on mitochondrial activity, reactive oxygen species (ROS) production, glutathione metabolism, and selected markers of oxidative/nitrosative stress in the periosteum-like tissue of patients treated with mandible fractures. The study group consisted of 30 patients with bilateral fractures of the mandible body eligible for surgical treatment. Our study is the first one that indicates disturbances of mitochondrial activity as well as a higher production of ROS in the periosteum-like tissue covering titanium fixations of the mandible. We also found significantly higher levels of reduced glutathione and enhanced activity of glutathione reductase in the periosteum homogenates of patients in the study group compared to the control group. Levels of nitrosative (S-nitrosothiols, peroxynitrite, nitrotyrosine) and oxidative stress biomarkers (malondialdehyde, protein carbonyls, dityrosine, kynurenine, and N-formylkynurenine) were statistically elevated in periosteum-like tissue covering titanium fixations. Although exposure to titanium fixations induces local antioxidant mechanisms, patients suffer oxidative damage, and in the periosteum-like tissue the phenomenon of metallosis was observed. Titanium implants cause oxidative/nitrosative stress as well as disturbances in mitochondrial activity. MDPI 2019-01-21 /pmc/articles/PMC6352110/ /pubmed/30669680 http://dx.doi.org/10.3390/jcm8010127 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borys, Jan
Maciejczyk, Mateusz
Antonowicz, Bożena
Krętowski, Adam
Sidun, Jarosław
Domel, Emilia
Dąbrowski, Jan Ryszard
Ładny, Jerzy Robert
Morawska, Katarzyna
Zalewska, Anna
Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title_full Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title_fullStr Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title_full_unstemmed Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title_short Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures
title_sort glutathione metabolism, mitochondria activity, and nitrosative stress in patients treated for mandible fractures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352110/
https://www.ncbi.nlm.nih.gov/pubmed/30669680
http://dx.doi.org/10.3390/jcm8010127
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