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Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity
Deoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold’...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352133/ https://www.ncbi.nlm.nih.gov/pubmed/30583578 http://dx.doi.org/10.3390/foods8010007 |
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author | Cambaza, Edgar Koseki, Shigenobu Kawamura, Shuso |
author_facet | Cambaza, Edgar Koseki, Shigenobu Kawamura, Shuso |
author_sort | Cambaza, Edgar |
collection | PubMed |
description | Deoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold’s color change and the quantity of DON synthesized throughout its lifecycle. Furthermore, a(w) has been found to affect the amount of DON. This study aimed to analyze the potential of F. graminearum surface color as a predictor of DON concentration at a(w) = 0.94, 0.97, and 0.99. Thus, 36 specimens were incubated at 25 °C, 12 at each a(w). After 4, 8, 12, and 16 days, three specimens from each a(w) were collected for color analysis and DON quantification. For color analysis, photos were taken and red, green and blue (RGB) channels were measured on ImageJ software. DON was quantified through liquid chromatography (HPLC). Color changes were only observed at a(w) = 0.99 because at lower a(w) the molds presented high growth of white mycelium. Yet, DON increased in all cases. It was only possible to relate the colors with DON concentration at a(w) = 0.99, where they presented inverse proportionality. |
format | Online Article Text |
id | pubmed-6352133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63521332019-02-01 Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity Cambaza, Edgar Koseki, Shigenobu Kawamura, Shuso Foods Article Deoxynivalenol (DON) is a well-known mycotoxin, responsible for outbreaks of gastrointestinal disorders in Japan. Fusarium graminearum, a parasite of cereal crops, produces this toxin and this is one of the reasons why it is important to understand its metabolism. It is possible to predict the mold’s color change and the quantity of DON synthesized throughout its lifecycle. Furthermore, a(w) has been found to affect the amount of DON. This study aimed to analyze the potential of F. graminearum surface color as a predictor of DON concentration at a(w) = 0.94, 0.97, and 0.99. Thus, 36 specimens were incubated at 25 °C, 12 at each a(w). After 4, 8, 12, and 16 days, three specimens from each a(w) were collected for color analysis and DON quantification. For color analysis, photos were taken and red, green and blue (RGB) channels were measured on ImageJ software. DON was quantified through liquid chromatography (HPLC). Color changes were only observed at a(w) = 0.99 because at lower a(w) the molds presented high growth of white mycelium. Yet, DON increased in all cases. It was only possible to relate the colors with DON concentration at a(w) = 0.99, where they presented inverse proportionality. MDPI 2018-12-23 /pmc/articles/PMC6352133/ /pubmed/30583578 http://dx.doi.org/10.3390/foods8010007 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cambaza, Edgar Koseki, Shigenobu Kawamura, Shuso Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title_full | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title_fullStr | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title_full_unstemmed | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title_short | Fusarium graminearum Colors and Deoxynivalenol Synthesis at Different Water Activity |
title_sort | fusarium graminearum colors and deoxynivalenol synthesis at different water activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352133/ https://www.ncbi.nlm.nih.gov/pubmed/30583578 http://dx.doi.org/10.3390/foods8010007 |
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