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Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.

A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion...

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Autores principales: Blesa, Alba, Sánchez, Mercedes, Sacristán-Horcajada, Eva, González-de la Fuente, Sandra, Peiró, Ramón, Berenguer, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352166/
https://www.ncbi.nlm.nih.gov/pubmed/30669685
http://dx.doi.org/10.3390/microorganisms7010025
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author Blesa, Alba
Sánchez, Mercedes
Sacristán-Horcajada, Eva
González-de la Fuente, Sandra
Peiró, Ramón
Berenguer, José
author_facet Blesa, Alba
Sánchez, Mercedes
Sacristán-Horcajada, Eva
González-de la Fuente, Sandra
Peiró, Ramón
Berenguer, José
author_sort Blesa, Alba
collection PubMed
description A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophiles.
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spelling pubmed-63521662019-02-01 Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp. Blesa, Alba Sánchez, Mercedes Sacristán-Horcajada, Eva González-de la Fuente, Sandra Peiró, Ramón Berenguer, José Microorganisms Article A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophiles. MDPI 2019-01-21 /pmc/articles/PMC6352166/ /pubmed/30669685 http://dx.doi.org/10.3390/microorganisms7010025 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blesa, Alba
Sánchez, Mercedes
Sacristán-Horcajada, Eva
González-de la Fuente, Sandra
Peiró, Ramón
Berenguer, José
Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title_full Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title_fullStr Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title_full_unstemmed Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title_short Into the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.
title_sort into the thermus mobilome: presence, diversity and recent activities of insertion sequences across thermus spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352166/
https://www.ncbi.nlm.nih.gov/pubmed/30669685
http://dx.doi.org/10.3390/microorganisms7010025
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