Cargando…

Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma

OBJECTIVES: Yttrium-90 (Y90)-radioembolisation (RE) significantly regresses locally advanced hepatocellular carcinoma and delays disease progression. The current study is designed to deeply interrogate the immunological impact of Y90-RE, which elicits a sustained therapeutic response. DESIGN: Time-o...

Descripción completa

Detalles Bibliográficos
Autores principales: Chew, Valerie, Lee, Yun Hua, Pan, Lu, Nasir, Nurul J M, Lim, Chun Jye, Chua, Camillus, Lai, Liyun, Hazirah, Sharifah Nur, Lim, Tony Kiat Hon, Goh, Brian K P, Chung, Alexander, Lo, Richard H G, Ng, David, Filarca, Rene L F, Albani, Salvatore, Chow, Pierce K H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352403/
https://www.ncbi.nlm.nih.gov/pubmed/29440463
http://dx.doi.org/10.1136/gutjnl-2017-315485
_version_ 1783390832172204032
author Chew, Valerie
Lee, Yun Hua
Pan, Lu
Nasir, Nurul J M
Lim, Chun Jye
Chua, Camillus
Lai, Liyun
Hazirah, Sharifah Nur
Lim, Tony Kiat Hon
Goh, Brian K P
Chung, Alexander
Lo, Richard H G
Ng, David
Filarca, Rene L F
Albani, Salvatore
Chow, Pierce K H
author_facet Chew, Valerie
Lee, Yun Hua
Pan, Lu
Nasir, Nurul J M
Lim, Chun Jye
Chua, Camillus
Lai, Liyun
Hazirah, Sharifah Nur
Lim, Tony Kiat Hon
Goh, Brian K P
Chung, Alexander
Lo, Richard H G
Ng, David
Filarca, Rene L F
Albani, Salvatore
Chow, Pierce K H
author_sort Chew, Valerie
collection PubMed
description OBJECTIVES: Yttrium-90 (Y90)-radioembolisation (RE) significantly regresses locally advanced hepatocellular carcinoma and delays disease progression. The current study is designed to deeply interrogate the immunological impact of Y90-RE, which elicits a sustained therapeutic response. DESIGN: Time-of-flight mass cytometry and next-generation sequencing (NGS) were used to analyse the immune landscapes of tumour-infiltrating leucocytes (TILs), tumour tissues and peripheral blood mononuclear cells (PBMCs) at different time points before and after Y90-RE. RESULTS: TILs isolated after Y90-RE exhibited signs of local immune activation: higher expression of granzyme B (GB) and infiltration of CD8(+) T cells, CD56(+) NK cells and CD8(+) CD56(+) NKT cells. NGS confirmed the upregulation of genes involved in innate and adaptive immune activation in Y90-RE-treated tumours. Chemotactic pathways involving CCL5 and CXCL16 correlated with the recruitment of activated GB(+)CD8(+) T cells to the Y90-RE-treated tumours. When comparing PBMCs before and after Y90-RE, we observed an increase in tumour necrosis factor-α on both the CD8(+) and CD4(+) T cells as well as an increase in percentage of antigen-presenting cells after Y90-RE, implying a systemic immune activation. Interestingly, a high percentage of PD-1(+)/Tim-3(+)CD8(+) T cells coexpressing the homing receptors CCR5 and CXCR6 denoted Y90-RE responders. A prediction model was also built to identify sustained responders to Y90-RE based on the immune profiles from pretreatment PBMCs. CONCLUSION: High-dimensional analysis of tumour and systemic immune landscapes identified local and systemic immune activation that corresponded to the sustained response to Y90-RE. Potential biomarkers associated with a positive clinical response were identified and a prediction model was built to identify sustained responders prior to treatment.
format Online
Article
Text
id pubmed-6352403
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BMJ Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-63524032019-02-21 Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma Chew, Valerie Lee, Yun Hua Pan, Lu Nasir, Nurul J M Lim, Chun Jye Chua, Camillus Lai, Liyun Hazirah, Sharifah Nur Lim, Tony Kiat Hon Goh, Brian K P Chung, Alexander Lo, Richard H G Ng, David Filarca, Rene L F Albani, Salvatore Chow, Pierce K H Gut Hepatology OBJECTIVES: Yttrium-90 (Y90)-radioembolisation (RE) significantly regresses locally advanced hepatocellular carcinoma and delays disease progression. The current study is designed to deeply interrogate the immunological impact of Y90-RE, which elicits a sustained therapeutic response. DESIGN: Time-of-flight mass cytometry and next-generation sequencing (NGS) were used to analyse the immune landscapes of tumour-infiltrating leucocytes (TILs), tumour tissues and peripheral blood mononuclear cells (PBMCs) at different time points before and after Y90-RE. RESULTS: TILs isolated after Y90-RE exhibited signs of local immune activation: higher expression of granzyme B (GB) and infiltration of CD8(+) T cells, CD56(+) NK cells and CD8(+) CD56(+) NKT cells. NGS confirmed the upregulation of genes involved in innate and adaptive immune activation in Y90-RE-treated tumours. Chemotactic pathways involving CCL5 and CXCL16 correlated with the recruitment of activated GB(+)CD8(+) T cells to the Y90-RE-treated tumours. When comparing PBMCs before and after Y90-RE, we observed an increase in tumour necrosis factor-α on both the CD8(+) and CD4(+) T cells as well as an increase in percentage of antigen-presenting cells after Y90-RE, implying a systemic immune activation. Interestingly, a high percentage of PD-1(+)/Tim-3(+)CD8(+) T cells coexpressing the homing receptors CCR5 and CXCR6 denoted Y90-RE responders. A prediction model was also built to identify sustained responders to Y90-RE based on the immune profiles from pretreatment PBMCs. CONCLUSION: High-dimensional analysis of tumour and systemic immune landscapes identified local and systemic immune activation that corresponded to the sustained response to Y90-RE. Potential biomarkers associated with a positive clinical response were identified and a prediction model was built to identify sustained responders prior to treatment. BMJ Publishing Group 2019-02 2018-02-13 /pmc/articles/PMC6352403/ /pubmed/29440463 http://dx.doi.org/10.1136/gutjnl-2017-315485 Text en © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2019. All rights reserved. No commercial use is permitted unless otherwise expressly granted. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Hepatology
Chew, Valerie
Lee, Yun Hua
Pan, Lu
Nasir, Nurul J M
Lim, Chun Jye
Chua, Camillus
Lai, Liyun
Hazirah, Sharifah Nur
Lim, Tony Kiat Hon
Goh, Brian K P
Chung, Alexander
Lo, Richard H G
Ng, David
Filarca, Rene L F
Albani, Salvatore
Chow, Pierce K H
Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title_full Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title_fullStr Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title_full_unstemmed Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title_short Immune activation underlies a sustained clinical response to Yttrium-90 radioembolisation in hepatocellular carcinoma
title_sort immune activation underlies a sustained clinical response to yttrium-90 radioembolisation in hepatocellular carcinoma
topic Hepatology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352403/
https://www.ncbi.nlm.nih.gov/pubmed/29440463
http://dx.doi.org/10.1136/gutjnl-2017-315485
work_keys_str_mv AT chewvalerie immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT leeyunhua immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT panlu immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT nasirnuruljm immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT limchunjye immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT chuacamillus immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT lailiyun immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT hazirahsharifahnur immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT limtonykiathon immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT gohbriankp immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT chungalexander immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT lorichardhg immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT ngdavid immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT filarcarenelf immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT albanisalvatore immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma
AT chowpiercekh immuneactivationunderliesasustainedclinicalresponsetoyttrium90radioembolisationinhepatocellularcarcinoma