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The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells

AIM: To investigate immunomodulatory activities of Picria fel-terrae Lour herbs extract against inflammatory biomarkers by conducting cell culture experiments. MATERIAL AND METHODS: The herbs of Picria fel-terrae Lour were dried and extracted with n-hexane, ethyl acetate, 96% ethanol, followed by ev...

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Autores principales: Auliafendri, Novycha, Rosidah, Yuandani, Suryani, Sri, Satria, Denny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352470/
https://www.ncbi.nlm.nih.gov/pubmed/30740154
http://dx.doi.org/10.3889/oamjms.2019.017
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author Auliafendri, Novycha
Rosidah,
Yuandani,
Suryani, Sri
Satria, Denny
author_facet Auliafendri, Novycha
Rosidah,
Yuandani,
Suryani, Sri
Satria, Denny
author_sort Auliafendri, Novycha
collection PubMed
description AIM: To investigate immunomodulatory activities of Picria fel-terrae Lour herbs extract against inflammatory biomarkers by conducting cell culture experiments. MATERIAL AND METHODS: The herbs of Picria fel-terrae Lour were dried and extracted with n-hexane, ethyl acetate, 96% ethanol, followed by evaporation and freeze-drying. Phytochemicals screening were analysed with thin layer chromatography method. Cell viability was assessed with MTT assay. The genes of Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, interleukin (IL)-1β and inducible Nitric Oxide Synthase (iNOS), Cyclooxygenase (COX-2) in lipopolysaccharide (LPS)-induced macrophages were analysed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULTS: Phytochemicals screening showed the presence of steroids in n-hexane extract (ENPFH) and flavonoids, glycosides, saponins, tannins in ethyl acetate (EEAPFH) and ethanol (EEPFH) extracts. The Viability of RAW 264.7 cell toward ENPFH, EEAPFH, and EEPFH (1-200 μgmL(-1)) showed no toxicity effects. At the gene level, ENPFH; EEAPFH; EEPFH decreased the gene expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 which induced with LPS (1 μgmL-1). CONCLUSIONS: Our results suggest that extracts of Picria fel-terrae Lour Herbs possesses immunomodulatory activities by inhibiting selected inflammatory biomarkers at the gene levels in LPS-induced macrophages.
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spelling pubmed-63524702019-02-08 The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells Auliafendri, Novycha Rosidah, Yuandani, Suryani, Sri Satria, Denny Open Access Maced J Med Sci Basic Science AIM: To investigate immunomodulatory activities of Picria fel-terrae Lour herbs extract against inflammatory biomarkers by conducting cell culture experiments. MATERIAL AND METHODS: The herbs of Picria fel-terrae Lour were dried and extracted with n-hexane, ethyl acetate, 96% ethanol, followed by evaporation and freeze-drying. Phytochemicals screening were analysed with thin layer chromatography method. Cell viability was assessed with MTT assay. The genes of Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, interleukin (IL)-1β and inducible Nitric Oxide Synthase (iNOS), Cyclooxygenase (COX-2) in lipopolysaccharide (LPS)-induced macrophages were analysed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULTS: Phytochemicals screening showed the presence of steroids in n-hexane extract (ENPFH) and flavonoids, glycosides, saponins, tannins in ethyl acetate (EEAPFH) and ethanol (EEPFH) extracts. The Viability of RAW 264.7 cell toward ENPFH, EEAPFH, and EEPFH (1-200 μgmL(-1)) showed no toxicity effects. At the gene level, ENPFH; EEAPFH; EEPFH decreased the gene expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 which induced with LPS (1 μgmL-1). CONCLUSIONS: Our results suggest that extracts of Picria fel-terrae Lour Herbs possesses immunomodulatory activities by inhibiting selected inflammatory biomarkers at the gene levels in LPS-induced macrophages. Republic of Macedonia 2019-01-05 /pmc/articles/PMC6352470/ /pubmed/30740154 http://dx.doi.org/10.3889/oamjms.2019.017 Text en Copyright: © 2019 Novycha Auliafendri, Rosidah, Yuandani, Sri Suryani, Denny Satria. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Auliafendri, Novycha
Rosidah,
Yuandani,
Suryani, Sri
Satria, Denny
The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title_full The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title_fullStr The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title_full_unstemmed The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title_short The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells
title_sort immunomodulatory activities of picria fel-terrae lour herbs towards raw 264.7 cells
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352470/
https://www.ncbi.nlm.nih.gov/pubmed/30740154
http://dx.doi.org/10.3889/oamjms.2019.017
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