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Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface

The bacterium Sporosarcina pasteurii (SP) is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation (MICP). We explored bacterial participation in the initial stages of the MICP process at the cellular length scale under two different growth environments...

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Autores principales: Ghosh, Tanushree, Bhaduri, Swayamdipta, Montemagno, Carlo, Kumar, Aloke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353136/
https://www.ncbi.nlm.nih.gov/pubmed/30699142
http://dx.doi.org/10.1371/journal.pone.0210339
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author Ghosh, Tanushree
Bhaduri, Swayamdipta
Montemagno, Carlo
Kumar, Aloke
author_facet Ghosh, Tanushree
Bhaduri, Swayamdipta
Montemagno, Carlo
Kumar, Aloke
author_sort Ghosh, Tanushree
collection PubMed
description The bacterium Sporosarcina pasteurii (SP) is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation (MICP). We explored bacterial participation in the initial stages of the MICP process at the cellular length scale under two different growth environments (a) liquid culture (b) MICP in a soft agar (0.5%) column. In the liquid culture, ex-situ imaging of the cellular environment indicated that S. pasteurii was facilitating nucleation of nanoscale crystals of calcium carbonate on bacterial cell surface and its growth via ureolysis. During the same period, the meso-scale environment (bulk medium) was found to have overgrown calcium carbonate crystals. The effect of media components (urea, CaCl(2)), presence of live and dead in the growth medium were explored. The agar column method allows for in-situ visualization of the phenomena, and using this platform, we found conclusive evidence of the bacterial cell surface facilitating formation of nanoscale crystals in the microenvironment. Here also the bulk environment or the meso-scale environment was found to possess overgrown calcium carbonate crystals. Extensive elemental analysis using Energy dispersive X-ray spectroscopy (EDS) and X-ray powder diffraction (XRD), confirmed that the crystals to be calcium carbonate, and two different polymorphs (calcite and vaterite) were identified. Active participation of S. pasteurii cell surface as the site of calcium carbonate precipitation has been shown using EDS elemental mapping with Scanning transmission electron microscopy (STEM) and scanning electron microscopy (SEM).
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spelling pubmed-63531362019-02-15 Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface Ghosh, Tanushree Bhaduri, Swayamdipta Montemagno, Carlo Kumar, Aloke PLoS One Research Article The bacterium Sporosarcina pasteurii (SP) is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation (MICP). We explored bacterial participation in the initial stages of the MICP process at the cellular length scale under two different growth environments (a) liquid culture (b) MICP in a soft agar (0.5%) column. In the liquid culture, ex-situ imaging of the cellular environment indicated that S. pasteurii was facilitating nucleation of nanoscale crystals of calcium carbonate on bacterial cell surface and its growth via ureolysis. During the same period, the meso-scale environment (bulk medium) was found to have overgrown calcium carbonate crystals. The effect of media components (urea, CaCl(2)), presence of live and dead in the growth medium were explored. The agar column method allows for in-situ visualization of the phenomena, and using this platform, we found conclusive evidence of the bacterial cell surface facilitating formation of nanoscale crystals in the microenvironment. Here also the bulk environment or the meso-scale environment was found to possess overgrown calcium carbonate crystals. Extensive elemental analysis using Energy dispersive X-ray spectroscopy (EDS) and X-ray powder diffraction (XRD), confirmed that the crystals to be calcium carbonate, and two different polymorphs (calcite and vaterite) were identified. Active participation of S. pasteurii cell surface as the site of calcium carbonate precipitation has been shown using EDS elemental mapping with Scanning transmission electron microscopy (STEM) and scanning electron microscopy (SEM). Public Library of Science 2019-01-30 /pmc/articles/PMC6353136/ /pubmed/30699142 http://dx.doi.org/10.1371/journal.pone.0210339 Text en © 2019 Ghosh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ghosh, Tanushree
Bhaduri, Swayamdipta
Montemagno, Carlo
Kumar, Aloke
Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title_full Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title_fullStr Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title_full_unstemmed Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title_short Sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
title_sort sporosarcina pasteurii can form nanoscale calcium carbonate crystals on cell surface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353136/
https://www.ncbi.nlm.nih.gov/pubmed/30699142
http://dx.doi.org/10.1371/journal.pone.0210339
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