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A role for methanogens and methane in the regulation of GLP‐1

INTRODUCTION: The gastrointestinal (GI) microbiome has emerged as a potential regulator of metabolism. However, the precise mechanisms of how microorganisms may influence physiology remain largely unknown. Interestingly, GI microorganisms, including methanogens, are localized within the same regions...

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Autores principales: Laverdure, Rose, Mezouari, Ania, Carson, Michael A., Basiliko, Nathan, Gagnon, Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353219/
https://www.ncbi.nlm.nih.gov/pubmed/30815543
http://dx.doi.org/10.1002/edm2.6
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author Laverdure, Rose
Mezouari, Ania
Carson, Michael A.
Basiliko, Nathan
Gagnon, Jeffrey
author_facet Laverdure, Rose
Mezouari, Ania
Carson, Michael A.
Basiliko, Nathan
Gagnon, Jeffrey
author_sort Laverdure, Rose
collection PubMed
description INTRODUCTION: The gastrointestinal (GI) microbiome has emerged as a potential regulator of metabolism. However, the precise mechanisms of how microorganisms may influence physiology remain largely unknown. Interestingly, GI microorganisms, including methanogens, are localized within the same regions as the glucagon‐like peptide‐1 (GLP‐1) secreting L cells. GLP‐1 plays key roles appetite and glucose regulation. Furthermore, both methane and GLP‐1 levels are altered in obese humans with metabolic disease. We predict that high‐fat diet‐induced obesity alters the abundance of GI methanogens and that methane may play a role in the GLP‐1 secretory response from the L cell. METHODS: To demonstrate this, GLP‐1 secretion response and faecal methanogens were examined in mice given a high‐fat diet for 14 weeks. In addition, the direct effect of methane on GLP‐1 secretion was assessed in two L‐cell models (NCI‐H716 and GLUTag). RESULTS: High‐fat diet caused a significant increase in both GLP‐1 secretion and faecal methanogen content. There was a direct correlation between GLP‐1 secretion response and faecal methanogen levels. In L cells, methane stimulated GLP‐1 secretion and enhanced intracellular cAMP content. CONCLUSION: These results indicate that alterations in the methanogen communities occurring in obesity may play a vital role in directly enhancing GLP‐1 secretion, and that methane can directly stimulate the secretion of GLP‐1.
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spelling pubmed-63532192019-02-27 A role for methanogens and methane in the regulation of GLP‐1 Laverdure, Rose Mezouari, Ania Carson, Michael A. Basiliko, Nathan Gagnon, Jeffrey Endocrinol Diabetes Metab Original Articles INTRODUCTION: The gastrointestinal (GI) microbiome has emerged as a potential regulator of metabolism. However, the precise mechanisms of how microorganisms may influence physiology remain largely unknown. Interestingly, GI microorganisms, including methanogens, are localized within the same regions as the glucagon‐like peptide‐1 (GLP‐1) secreting L cells. GLP‐1 plays key roles appetite and glucose regulation. Furthermore, both methane and GLP‐1 levels are altered in obese humans with metabolic disease. We predict that high‐fat diet‐induced obesity alters the abundance of GI methanogens and that methane may play a role in the GLP‐1 secretory response from the L cell. METHODS: To demonstrate this, GLP‐1 secretion response and faecal methanogens were examined in mice given a high‐fat diet for 14 weeks. In addition, the direct effect of methane on GLP‐1 secretion was assessed in two L‐cell models (NCI‐H716 and GLUTag). RESULTS: High‐fat diet caused a significant increase in both GLP‐1 secretion and faecal methanogen content. There was a direct correlation between GLP‐1 secretion response and faecal methanogen levels. In L cells, methane stimulated GLP‐1 secretion and enhanced intracellular cAMP content. CONCLUSION: These results indicate that alterations in the methanogen communities occurring in obesity may play a vital role in directly enhancing GLP‐1 secretion, and that methane can directly stimulate the secretion of GLP‐1. John Wiley and Sons Inc. 2017-12-01 /pmc/articles/PMC6353219/ /pubmed/30815543 http://dx.doi.org/10.1002/edm2.6 Text en © 2017 The Authors. Endocrinology, Diabetes & Metabolism published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Laverdure, Rose
Mezouari, Ania
Carson, Michael A.
Basiliko, Nathan
Gagnon, Jeffrey
A role for methanogens and methane in the regulation of GLP‐1
title A role for methanogens and methane in the regulation of GLP‐1
title_full A role for methanogens and methane in the regulation of GLP‐1
title_fullStr A role for methanogens and methane in the regulation of GLP‐1
title_full_unstemmed A role for methanogens and methane in the regulation of GLP‐1
title_short A role for methanogens and methane in the regulation of GLP‐1
title_sort role for methanogens and methane in the regulation of glp‐1
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353219/
https://www.ncbi.nlm.nih.gov/pubmed/30815543
http://dx.doi.org/10.1002/edm2.6
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