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Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain

A gene encoding chitinase from B. subtilis has been isolated after optimization of PCR conditions. It was cloned with two different prometers, T7 promoter of the pJET1.2/blunt cloning vector and the SP6 promoter of pGEM®-T Easy vector. After transforming E. coli DH5α, two transformants were selected...

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Detalles Bibliográficos
Autores principales:	Abdel-Salam, Mohamed S., Ameen, Hoda H., Kassab, Abdallah S.M., Mahgoob, Ahmed E.A., Elkelany, Usama S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Academy of Scientific Research and Technology, Egypt 2018
Materias:	Microbial/industrial Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353750/ https://www.ncbi.nlm.nih.gov/pubmed/30733739 http://dx.doi.org/10.1016/j.jgeb.2018.05.006

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