High level expression and purification of recombinant flounder growth hormone in E. coli

Recombinant flounder growth hormone was overproduced in E. coli by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into E. coli BL21 (DE3). Induction at lower temperature, lower IPT...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Tae-Jin, Geletu, Temesgen Tola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2018
Materias:
Microbial/industrial Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353774/
https://www.ncbi.nlm.nih.gov/pubmed/30733745
http://dx.doi.org/10.1016/j.jgeb.2018.03.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353774/
https://www.ncbi.nlm.nih.gov/pubmed/30733745
http://dx.doi.org/10.1016/j.jgeb.2018.03.006

Ejemplares similares