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High level expression and purification of recombinant flounder growth hormone in E. coli
Recombinant flounder growth hormone was overproduced in E. coli by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into E. coli BL21 (DE3). Induction at lower temperature, lower IPT...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academy of Scientific Research and Technology, Egypt
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353774/ https://www.ncbi.nlm.nih.gov/pubmed/30733745 http://dx.doi.org/10.1016/j.jgeb.2018.03.006 |