Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis

The currently available haemoglobin A1c (HbA1c) enzymatic assay consists of two specific steps: proteolysis of HbA1c and oxidation of the liberated fructosyl peptide by fructosyl peptide oxidase (FPOX). To develop a more convenient and high throughput assay, we devised novel protease-free assay syst...

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Detalles Bibliográficos
Autores principales: Ogawa, Noriyuki, Kimura, Takehide, Umehara, Fumi, Katayama, Yuki, Nagai, Go, Suzuki, Keiko, Aisaka, Kazuo, Maruyama, Yukie, Itoh, Takafumi, Hashimoto, Wataru, Murata, Kousaku, Ichimura, Michio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353924/
https://www.ncbi.nlm.nih.gov/pubmed/30700768
http://dx.doi.org/10.1038/s41598-018-37806-x

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