Cargando…

G9a-dependent histone methylation can be induced in G1 phase of cell cycle

Epigenetic information (epigenome) on chromatin is crucial for the determination of cellular identity and for the expression of cell type-specific biological functions. The cell type-specific epigenome is maintained beyond replication and cell division. Nucleosomes of chromatin just after DNA replic...

Descripción completa

Detalles Bibliográficos
Autores principales: Fukuda, Mikiko, Sakaue-Sawano, Asako, Shimura, Chikako, Tachibana, Makoto, Miyawaki, Atsushi, Shinkai, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354049/
https://www.ncbi.nlm.nih.gov/pubmed/30700744
http://dx.doi.org/10.1038/s41598-018-37507-5
_version_ 1783391100710420480
author Fukuda, Mikiko
Sakaue-Sawano, Asako
Shimura, Chikako
Tachibana, Makoto
Miyawaki, Atsushi
Shinkai, Yoichi
author_facet Fukuda, Mikiko
Sakaue-Sawano, Asako
Shimura, Chikako
Tachibana, Makoto
Miyawaki, Atsushi
Shinkai, Yoichi
author_sort Fukuda, Mikiko
collection PubMed
description Epigenetic information (epigenome) on chromatin is crucial for the determination of cellular identity and for the expression of cell type-specific biological functions. The cell type-specific epigenome is maintained beyond replication and cell division. Nucleosomes of chromatin just after DNA replication are a mixture of old histones with the parental epigenome and newly synthesized histones without such information. The diluted epigenome is mostly restored within one cell cycle using the epigenome on the parental DNA and nucleosomes as replication templates. However, many important questions about the epigenome replication process remain to be clarified. In this study, we investigated the model system comprising of dimethylated histone H3 lysine 9 (H3K9me2) and its regulation by the lysine methyltransferase G9a. Using this epigenome model system, we addressed whether H3K9me2 can be induced in specific cell cycle stages, especially G1. Using cell cycle-specific degrons, we achieved G1 or late G1-to M phases specific accumulation of exogenous G9a in G9a deficient cells. Importantly, global levels of H3K9me2 were significantly recovered by both cell types. These data indicate that H3K9me2 may be plastic and inducible, even in the long-living, terminally-differentiated, post-mitotic, G0-G1 cell population in vivo. This knowledge is valuable in designing epigenome-manipulation-based treatments for diseases.
format Online
Article
Text
id pubmed-6354049
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-63540492019-02-01 G9a-dependent histone methylation can be induced in G1 phase of cell cycle Fukuda, Mikiko Sakaue-Sawano, Asako Shimura, Chikako Tachibana, Makoto Miyawaki, Atsushi Shinkai, Yoichi Sci Rep Article Epigenetic information (epigenome) on chromatin is crucial for the determination of cellular identity and for the expression of cell type-specific biological functions. The cell type-specific epigenome is maintained beyond replication and cell division. Nucleosomes of chromatin just after DNA replication are a mixture of old histones with the parental epigenome and newly synthesized histones without such information. The diluted epigenome is mostly restored within one cell cycle using the epigenome on the parental DNA and nucleosomes as replication templates. However, many important questions about the epigenome replication process remain to be clarified. In this study, we investigated the model system comprising of dimethylated histone H3 lysine 9 (H3K9me2) and its regulation by the lysine methyltransferase G9a. Using this epigenome model system, we addressed whether H3K9me2 can be induced in specific cell cycle stages, especially G1. Using cell cycle-specific degrons, we achieved G1 or late G1-to M phases specific accumulation of exogenous G9a in G9a deficient cells. Importantly, global levels of H3K9me2 were significantly recovered by both cell types. These data indicate that H3K9me2 may be plastic and inducible, even in the long-living, terminally-differentiated, post-mitotic, G0-G1 cell population in vivo. This knowledge is valuable in designing epigenome-manipulation-based treatments for diseases. Nature Publishing Group UK 2019-01-30 /pmc/articles/PMC6354049/ /pubmed/30700744 http://dx.doi.org/10.1038/s41598-018-37507-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fukuda, Mikiko
Sakaue-Sawano, Asako
Shimura, Chikako
Tachibana, Makoto
Miyawaki, Atsushi
Shinkai, Yoichi
G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title_full G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title_fullStr G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title_full_unstemmed G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title_short G9a-dependent histone methylation can be induced in G1 phase of cell cycle
title_sort g9a-dependent histone methylation can be induced in g1 phase of cell cycle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354049/
https://www.ncbi.nlm.nih.gov/pubmed/30700744
http://dx.doi.org/10.1038/s41598-018-37507-5
work_keys_str_mv AT fukudamikiko g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle
AT sakauesawanoasako g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle
AT shimurachikako g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle
AT tachibanamakoto g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle
AT miyawakiatsushi g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle
AT shinkaiyoichi g9adependenthistonemethylationcanbeinduceding1phaseofcellcycle