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Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers
Cyclospora cayetanensis is a human parasite transmitted via ingestion of contaminated food or water. Cases of C. cayetanensis infection acquired in the United States often go unexplained, partly because of the difficulties associated with epidemiologic investigations of such cases and the lack of ge...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354607/ https://www.ncbi.nlm.nih.gov/pubmed/30702060 http://dx.doi.org/10.1051/parasite/2019004 |
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author | Hofstetter, Jessica N. Nascimento, Fernanda S. Park, Subin Casillas, Shannon Herwaldt, Barbara L. Arrowood, Michael J. Qvarnstrom, Yvonne |
author_facet | Hofstetter, Jessica N. Nascimento, Fernanda S. Park, Subin Casillas, Shannon Herwaldt, Barbara L. Arrowood, Michael J. Qvarnstrom, Yvonne |
author_sort | Hofstetter, Jessica N. |
collection | PubMed |
description | Cyclospora cayetanensis is a human parasite transmitted via ingestion of contaminated food or water. Cases of C. cayetanensis infection acquired in the United States often go unexplained, partly because of the difficulties associated with epidemiologic investigations of such cases and the lack of genotyping methods. A Multilocus Sequence Typing (MLST) method for C. cayetanensis based on five microsatellite loci amplified by nested PCR was described in 2016. The MLST loci had high variability, but many specimens could not be assigned a type because of poor DNA sequencing quality at one or more loci. We analyzed Cyclospora-positive stool specimens collected during 1997–2016 from 54 patients, including 51 from the United States. We noted limited inter-specimen variability for one locus (CYC15) and the frequent occurrence of unreadable DNA sequences for two loci (CYC3 and CYC13). Overall, using the remaining two loci (CYC21 and CYC22), we detected 17 different concatenated sequence types. For four of five clusters of epidemiologically linked cases for which we had specimens from >1 case-patient, the specimens associated with the same cluster had the same type. However, we also noted the same type for specimens that were geographically and temporally unrelated, indicating poor discriminatory power. Furthermore, many specimens had what appeared to be a mixture of sequence types at locus CYC22. We conclude that it may be difficult to substantially improve the performance of the MLST method because of the nucleotide repeat features of the markers, along with the frequent occurrence of mixed genotypes in Cyclospora infections. |
format | Online Article Text |
id | pubmed-6354607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-63546072019-02-15 Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers Hofstetter, Jessica N. Nascimento, Fernanda S. Park, Subin Casillas, Shannon Herwaldt, Barbara L. Arrowood, Michael J. Qvarnstrom, Yvonne Parasite Research Article Cyclospora cayetanensis is a human parasite transmitted via ingestion of contaminated food or water. Cases of C. cayetanensis infection acquired in the United States often go unexplained, partly because of the difficulties associated with epidemiologic investigations of such cases and the lack of genotyping methods. A Multilocus Sequence Typing (MLST) method for C. cayetanensis based on five microsatellite loci amplified by nested PCR was described in 2016. The MLST loci had high variability, but many specimens could not be assigned a type because of poor DNA sequencing quality at one or more loci. We analyzed Cyclospora-positive stool specimens collected during 1997–2016 from 54 patients, including 51 from the United States. We noted limited inter-specimen variability for one locus (CYC15) and the frequent occurrence of unreadable DNA sequences for two loci (CYC3 and CYC13). Overall, using the remaining two loci (CYC21 and CYC22), we detected 17 different concatenated sequence types. For four of five clusters of epidemiologically linked cases for which we had specimens from >1 case-patient, the specimens associated with the same cluster had the same type. However, we also noted the same type for specimens that were geographically and temporally unrelated, indicating poor discriminatory power. Furthermore, many specimens had what appeared to be a mixture of sequence types at locus CYC22. We conclude that it may be difficult to substantially improve the performance of the MLST method because of the nucleotide repeat features of the markers, along with the frequent occurrence of mixed genotypes in Cyclospora infections. EDP Sciences 2019-01-31 /pmc/articles/PMC6354607/ /pubmed/30702060 http://dx.doi.org/10.1051/parasite/2019004 Text en © J.N. Hofstetter et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hofstetter, Jessica N. Nascimento, Fernanda S. Park, Subin Casillas, Shannon Herwaldt, Barbara L. Arrowood, Michael J. Qvarnstrom, Yvonne Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title | Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title_full | Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title_fullStr | Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title_full_unstemmed | Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title_short | Evaluation of Multilocus Sequence Typing of Cyclospora cayetanensis based on microsatellite markers |
title_sort | evaluation of multilocus sequence typing of cyclospora cayetanensis based on microsatellite markers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354607/ https://www.ncbi.nlm.nih.gov/pubmed/30702060 http://dx.doi.org/10.1051/parasite/2019004 |
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