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Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor

BACKGROUND: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion. OBJECTIVES: In the pres...

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Autores principales: Karakuş, Fuat, Eyol, Ergül, Yılmaz, Kadir, Ünüvar, Songül
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354875/
https://www.ncbi.nlm.nih.gov/pubmed/30766596
http://dx.doi.org/10.4314/ahs.v18i4.51
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author Karakuş, Fuat
Eyol, Ergül
Yılmaz, Kadir
Ünüvar, Songül
author_facet Karakuş, Fuat
Eyol, Ergül
Yılmaz, Kadir
Ünüvar, Songül
author_sort Karakuş, Fuat
collection PubMed
description BACKGROUND: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion. OBJECTIVES: In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells. METHODS: Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells. RESULTS: Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 µM dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 µM concentrations. CONCLUSION: Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium.
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spelling pubmed-63548752019-02-14 Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor Karakuş, Fuat Eyol, Ergül Yılmaz, Kadir Ünüvar, Songül Afr Health Sci Articles BACKGROUND: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion. OBJECTIVES: In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells. METHODS: Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells. RESULTS: Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 µM dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 µM concentrations. CONCLUSION: Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium. Makerere Medical School 2018-12 /pmc/articles/PMC6354875/ /pubmed/30766596 http://dx.doi.org/10.4314/ahs.v18i4.51 Text en © 2018 Karakuş et al. Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Karakuş, Fuat
Eyol, Ergül
Yılmaz, Kadir
Ünüvar, Songül
Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title_full Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title_fullStr Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title_full_unstemmed Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title_short Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor
title_sort inhibition of cell proliferation, migration and colony formation of ls174t cells by carbonic anhydrase inhibitor
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354875/
https://www.ncbi.nlm.nih.gov/pubmed/30766596
http://dx.doi.org/10.4314/ahs.v18i4.51
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