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Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses
Defining and loading of immunogenic and safe cancer antigens remain a major challenge for designing dendritic cell (DC)-based cancer vaccines. In this study, we defined a prototype strategy of using DC-based vaccines pulsed with placenta-derived heat shock protein gp96 to induces anti-tumor T cell r...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354997/ https://www.ncbi.nlm.nih.gov/pubmed/30703157 http://dx.doi.org/10.1371/journal.pone.0211490 |
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author | Zheng, Huaguo Liu, Lanlan Zhang, Han Kan, Fangming Wang, Shuo Li, Yang Tian, Huaqin Meng, Songdong |
author_facet | Zheng, Huaguo Liu, Lanlan Zhang, Han Kan, Fangming Wang, Shuo Li, Yang Tian, Huaqin Meng, Songdong |
author_sort | Zheng, Huaguo |
collection | PubMed |
description | Defining and loading of immunogenic and safe cancer antigens remain a major challenge for designing dendritic cell (DC)-based cancer vaccines. In this study, we defined a prototype strategy of using DC-based vaccines pulsed with placenta-derived heat shock protein gp96 to induces anti-tumor T cell responses. Placental gp96 was efficiently taken up by CD11c+ bone marrow-derived DCs (BMDCs) and resulted in moderate BMDC maturation. Splenocytes and cytotoxic T cells (CTLs) generated with mouse BMDCs pulsed with placental gp96 specifically lysed B16 melanoma and LLC lung carcinoma cells. In both transplantable melanoma and lung carcinoma mice models, immunization with placental gp96-stimulated BMDCs led to a significant decrease in tumor growth and mouse mortality with respect to mice treated with liver gp96-pulsed BMDCs or placental gp96 alone. This vaccine induced strong cross-reactive tumor-specific T cell responses. Our results revealed that DCs pulsed with placenta-derived gp96 represent an effective immunotherapy to induce tumor-reactive immune responses, possibly via loading DCs with its associated carcinoembryonic antigens. |
format | Online Article Text |
id | pubmed-6354997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63549972019-02-15 Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses Zheng, Huaguo Liu, Lanlan Zhang, Han Kan, Fangming Wang, Shuo Li, Yang Tian, Huaqin Meng, Songdong PLoS One Research Article Defining and loading of immunogenic and safe cancer antigens remain a major challenge for designing dendritic cell (DC)-based cancer vaccines. In this study, we defined a prototype strategy of using DC-based vaccines pulsed with placenta-derived heat shock protein gp96 to induces anti-tumor T cell responses. Placental gp96 was efficiently taken up by CD11c+ bone marrow-derived DCs (BMDCs) and resulted in moderate BMDC maturation. Splenocytes and cytotoxic T cells (CTLs) generated with mouse BMDCs pulsed with placental gp96 specifically lysed B16 melanoma and LLC lung carcinoma cells. In both transplantable melanoma and lung carcinoma mice models, immunization with placental gp96-stimulated BMDCs led to a significant decrease in tumor growth and mouse mortality with respect to mice treated with liver gp96-pulsed BMDCs or placental gp96 alone. This vaccine induced strong cross-reactive tumor-specific T cell responses. Our results revealed that DCs pulsed with placenta-derived gp96 represent an effective immunotherapy to induce tumor-reactive immune responses, possibly via loading DCs with its associated carcinoembryonic antigens. Public Library of Science 2019-01-31 /pmc/articles/PMC6354997/ /pubmed/30703157 http://dx.doi.org/10.1371/journal.pone.0211490 Text en © 2019 Zheng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zheng, Huaguo Liu, Lanlan Zhang, Han Kan, Fangming Wang, Shuo Li, Yang Tian, Huaqin Meng, Songdong Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title | Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title_full | Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title_fullStr | Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title_full_unstemmed | Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title_short | Dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
title_sort | dendritic cells pulsed with placental gp96 promote tumor-reactive immune responses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354997/ https://www.ncbi.nlm.nih.gov/pubmed/30703157 http://dx.doi.org/10.1371/journal.pone.0211490 |
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