Reprogramming the antigen specificity of B cells using genome-editing technologies

We have developed a method to introduce novel paratopes into the human antibody repertoire by modifying the immunoglobulin (Ig) genes of mature B cells directly using genome editing technologies. We used CRISPR-Cas9 in a homology directed repair strategy, to replace the heavy chain (HC) variable reg...

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Detalles Bibliográficos
Autores principales: Voss, James E, Gonzalez-Martin, Alicia, Andrabi, Raiees, Fuller, Roberta P, Murrell, Ben, McCoy, Laura E, Porter, Katelyn, Huang, Deli, Li, Wenjuan, Sok, Devin, Le, Khoa, Briney, Bryan, Chateau, Morgan, Rogers, Geoffrey, Hangartner, Lars, Feeney, Ann J, Nemazee, David, Cannon, Paula, Burton, Dennis R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Immunology and Inflammation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355199/
https://www.ncbi.nlm.nih.gov/pubmed/30648968
http://dx.doi.org/10.7554/eLife.42995

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355199/
https://www.ncbi.nlm.nih.gov/pubmed/30648968
http://dx.doi.org/10.7554/eLife.42995

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