Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus

A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transc...

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Detalles Bibliográficos
Autores principales: Ye, Meiping, Xu, Liyun, Zou, Yuzhen, Li, Bing, Guo, Qi, Zhang, Yongjie, Zhan, Mengling, Xu, Benyong, Yu, Fangyou, Zhang, Zhemin, Chu, Haiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355594/
https://www.ncbi.nlm.nih.gov/pubmed/30478161
http://dx.doi.org/10.1128/AAC.01842-18
Descripción
Sumario:A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transcriptional levels of efflux pumps lmrS and mmpL9 in LZD-resistant isolates. Genome comparative analysis identified several new LZD resistance-associated genes. This study highlights the role of efflux pumps in LZD-resistant M. abscessus and proposes potential target genes for further studies.

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