Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus

A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transc...

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Autores principales: Ye, Meiping, Xu, Liyun, Zou, Yuzhen, Li, Bing, Guo, Qi, Zhang, Yongjie, Zhan, Mengling, Xu, Benyong, Yu, Fangyou, Zhang, Zhemin, Chu, Haiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355594/
https://www.ncbi.nlm.nih.gov/pubmed/30478161
http://dx.doi.org/10.1128/AAC.01842-18
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author Ye, Meiping
Xu, Liyun
Zou, Yuzhen
Li, Bing
Guo, Qi
Zhang, Yongjie
Zhan, Mengling
Xu, Benyong
Yu, Fangyou
Zhang, Zhemin
Chu, Haiqing
author_facet Ye, Meiping
Xu, Liyun
Zou, Yuzhen
Li, Bing
Guo, Qi
Zhang, Yongjie
Zhan, Mengling
Xu, Benyong
Yu, Fangyou
Zhang, Zhemin
Chu, Haiqing
author_sort Ye, Meiping
collection PubMed
description A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transcriptional levels of efflux pumps lmrS and mmpL9 in LZD-resistant isolates. Genome comparative analysis identified several new LZD resistance-associated genes. This study highlights the role of efflux pumps in LZD-resistant M. abscessus and proposes potential target genes for further studies.
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spelling pubmed-63555942019-02-01 Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus Ye, Meiping Xu, Liyun Zou, Yuzhen Li, Bing Guo, Qi Zhang, Yongjie Zhan, Mengling Xu, Benyong Yu, Fangyou Zhang, Zhemin Chu, Haiqing Antimicrob Agents Chemother Mechanisms of Resistance A total of 194 Mycobacterium abscessus isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transcriptional levels of efflux pumps lmrS and mmpL9 in LZD-resistant isolates. Genome comparative analysis identified several new LZD resistance-associated genes. This study highlights the role of efflux pumps in LZD-resistant M. abscessus and proposes potential target genes for further studies. American Society for Microbiology 2019-01-29 /pmc/articles/PMC6355594/ /pubmed/30478161 http://dx.doi.org/10.1128/AAC.01842-18 Text en Copyright © 2019 Ye et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mechanisms of Resistance
Ye, Meiping
Xu, Liyun
Zou, Yuzhen
Li, Bing
Guo, Qi
Zhang, Yongjie
Zhan, Mengling
Xu, Benyong
Yu, Fangyou
Zhang, Zhemin
Chu, Haiqing
Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title_full Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title_fullStr Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title_full_unstemmed Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title_short Molecular Analysis of Linezolid-Resistant Clinical Isolates of Mycobacterium abscessus
title_sort molecular analysis of linezolid-resistant clinical isolates of mycobacterium abscessus
topic Mechanisms of Resistance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355594/
https://www.ncbi.nlm.nih.gov/pubmed/30478161
http://dx.doi.org/10.1128/AAC.01842-18
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