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Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L.
Phloem plays a fundamental role in plants by transporting hormones, nutrients, proteins, RNAs, and carbohydrates essential for plant growth and development. However, the identity of the underlying phloem genes and pathways remain enigmatic especially in agriculturally important perennial crops, in p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355854/ https://www.ncbi.nlm.nih.gov/pubmed/30729006 http://dx.doi.org/10.1038/s41438-018-0092-4 |
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author | Collum, Tamara D. Lutton, Elizabeth Raines, C. Douglas Dardick, Christopher Culver, James N. |
author_facet | Collum, Tamara D. Lutton, Elizabeth Raines, C. Douglas Dardick, Christopher Culver, James N. |
author_sort | Collum, Tamara D. |
collection | PubMed |
description | Phloem plays a fundamental role in plants by transporting hormones, nutrients, proteins, RNAs, and carbohydrates essential for plant growth and development. However, the identity of the underlying phloem genes and pathways remain enigmatic especially in agriculturally important perennial crops, in part, due to the technical difficulty of phloem sampling. Here, we used two phloem-specific promoters and a translating ribosome affinity purification (TRAP) strategy to characterize the phloem translatome during leaf development at 2, 4, and 6 weeks post vernalization in plum (Prunus domestica L.). Results provide insight into the changing phloem processes that occur during leaf development. These processes included the early activation of DNA replication genes that are likely involved in phloem cell division during leaf expansion, as well as the upregulation of phloem genes associated with sink to source conversion, induction of defense processes, and signaling for reproduction. Combined these results reveal the dynamics of phloem gene expression during leaf development and establish the TRAP system as a powerful tool for studying phloem-specific functions and responses in trees. |
format | Online Article Text |
id | pubmed-6355854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63558542019-02-06 Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. Collum, Tamara D. Lutton, Elizabeth Raines, C. Douglas Dardick, Christopher Culver, James N. Hortic Res Article Phloem plays a fundamental role in plants by transporting hormones, nutrients, proteins, RNAs, and carbohydrates essential for plant growth and development. However, the identity of the underlying phloem genes and pathways remain enigmatic especially in agriculturally important perennial crops, in part, due to the technical difficulty of phloem sampling. Here, we used two phloem-specific promoters and a translating ribosome affinity purification (TRAP) strategy to characterize the phloem translatome during leaf development at 2, 4, and 6 weeks post vernalization in plum (Prunus domestica L.). Results provide insight into the changing phloem processes that occur during leaf development. These processes included the early activation of DNA replication genes that are likely involved in phloem cell division during leaf expansion, as well as the upregulation of phloem genes associated with sink to source conversion, induction of defense processes, and signaling for reproduction. Combined these results reveal the dynamics of phloem gene expression during leaf development and establish the TRAP system as a powerful tool for studying phloem-specific functions and responses in trees. Nature Publishing Group UK 2019-02-01 /pmc/articles/PMC6355854/ /pubmed/30729006 http://dx.doi.org/10.1038/s41438-018-0092-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Collum, Tamara D. Lutton, Elizabeth Raines, C. Douglas Dardick, Christopher Culver, James N. Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title | Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title_full | Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title_fullStr | Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title_full_unstemmed | Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title_short | Identification of phloem-associated translatome alterations during leaf development in Prunus domestica L. |
title_sort | identification of phloem-associated translatome alterations during leaf development in prunus domestica l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355854/ https://www.ncbi.nlm.nih.gov/pubmed/30729006 http://dx.doi.org/10.1038/s41438-018-0092-4 |
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