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Modular Design of Programmable Mechanofluorescent DNA Hydrogels

Mechanosensing systems are ubiquitous in nature and control many functions from cell spreading to wound healing. Biologic systems typically rely on supramolecular transformations and secondary reporter systems to sense weak forces. By contrast, synthetic mechanosensitive materials often use covalent...

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Autores principales: Merindol, Remi, Delechiave, Giovanne, Heinen, Laura, Catalani, Luiz Henrique, Walther, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355893/
https://www.ncbi.nlm.nih.gov/pubmed/30705271
http://dx.doi.org/10.1038/s41467-019-08428-2
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author Merindol, Remi
Delechiave, Giovanne
Heinen, Laura
Catalani, Luiz Henrique
Walther, Andreas
author_facet Merindol, Remi
Delechiave, Giovanne
Heinen, Laura
Catalani, Luiz Henrique
Walther, Andreas
author_sort Merindol, Remi
collection PubMed
description Mechanosensing systems are ubiquitous in nature and control many functions from cell spreading to wound healing. Biologic systems typically rely on supramolecular transformations and secondary reporter systems to sense weak forces. By contrast, synthetic mechanosensitive materials often use covalent transformations of chromophores, serving both as force sensor and reporter, which hinders orthogonal engineering of their sensitivity, response and modularity. Here, we introduce FRET-based, rationally tunable DNA tension probes into macroscopic 3D all-DNA hydrogels to prepare mechanofluorescent materials with programmable sacrificial bonds and stress relaxation. This design addresses current limitations of mechanochromic system by offering spatiotemporal resolution, as well as quantitative and modular force sensing in soft hydrogels. The programmable force probe design further grants temporal control over the recovery of the mechanofluorescence during stress relaxation, enabling reversible and irreversible strain sensing. We show proof-of-concept applications to study strain fields in composites and to visualize freezing-induced strain patterns in homogeneous hydrogels.
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spelling pubmed-63558932019-02-04 Modular Design of Programmable Mechanofluorescent DNA Hydrogels Merindol, Remi Delechiave, Giovanne Heinen, Laura Catalani, Luiz Henrique Walther, Andreas Nat Commun Article Mechanosensing systems are ubiquitous in nature and control many functions from cell spreading to wound healing. Biologic systems typically rely on supramolecular transformations and secondary reporter systems to sense weak forces. By contrast, synthetic mechanosensitive materials often use covalent transformations of chromophores, serving both as force sensor and reporter, which hinders orthogonal engineering of their sensitivity, response and modularity. Here, we introduce FRET-based, rationally tunable DNA tension probes into macroscopic 3D all-DNA hydrogels to prepare mechanofluorescent materials with programmable sacrificial bonds and stress relaxation. This design addresses current limitations of mechanochromic system by offering spatiotemporal resolution, as well as quantitative and modular force sensing in soft hydrogels. The programmable force probe design further grants temporal control over the recovery of the mechanofluorescence during stress relaxation, enabling reversible and irreversible strain sensing. We show proof-of-concept applications to study strain fields in composites and to visualize freezing-induced strain patterns in homogeneous hydrogels. Nature Publishing Group UK 2019-01-31 /pmc/articles/PMC6355893/ /pubmed/30705271 http://dx.doi.org/10.1038/s41467-019-08428-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Merindol, Remi
Delechiave, Giovanne
Heinen, Laura
Catalani, Luiz Henrique
Walther, Andreas
Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title_full Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title_fullStr Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title_full_unstemmed Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title_short Modular Design of Programmable Mechanofluorescent DNA Hydrogels
title_sort modular design of programmable mechanofluorescent dna hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355893/
https://www.ncbi.nlm.nih.gov/pubmed/30705271
http://dx.doi.org/10.1038/s41467-019-08428-2
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