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Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections
A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356340/ https://www.ncbi.nlm.nih.gov/pubmed/30669600 http://dx.doi.org/10.3390/v11010086 |
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author | Chen, Yibao Guo, Guanghao Sun, Erchao Song, Jiaoyang Yang, Lan Zhu, Lili Liang, Wan Hua, Lin Peng, Zhong Tang, Xibiao Chen, Huanchun Wu, Bin |
author_facet | Chen, Yibao Guo, Guanghao Sun, Erchao Song, Jiaoyang Yang, Lan Zhu, Lili Liang, Wan Hua, Lin Peng, Zhong Tang, Xibiao Chen, Huanchun Wu, Bin |
author_sort | Chen, Yibao |
collection | PubMed |
description | A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic P. multocida type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the first lytic phage specific for P. multocida type D sequenced thus far, presents a 37,287-bp double-stranded DNA genome with a 223-bp terminal redundancy. The PHB01 genome showed the highest homology with that of PHB02, a lytic phage specific for P. multocida type A. Phylogenetic analysis showed that PHB01 and PHB02 were composed of a genus that was close to the T7-virus genus. In vivo tests using mouse models showed that the administration of PHB01 was safe to the mice and had a good effect on treating the mice infected with different P. multocida type D strains including virulent strain HN05. These findings suggest that PHB01 has a potential use in therapy against infections caused by P. multocida type D. |
format | Online Article Text |
id | pubmed-6356340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63563402019-02-05 Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections Chen, Yibao Guo, Guanghao Sun, Erchao Song, Jiaoyang Yang, Lan Zhu, Lili Liang, Wan Hua, Lin Peng, Zhong Tang, Xibiao Chen, Huanchun Wu, Bin Viruses Article A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic P. multocida type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the first lytic phage specific for P. multocida type D sequenced thus far, presents a 37,287-bp double-stranded DNA genome with a 223-bp terminal redundancy. The PHB01 genome showed the highest homology with that of PHB02, a lytic phage specific for P. multocida type A. Phylogenetic analysis showed that PHB01 and PHB02 were composed of a genus that was close to the T7-virus genus. In vivo tests using mouse models showed that the administration of PHB01 was safe to the mice and had a good effect on treating the mice infected with different P. multocida type D strains including virulent strain HN05. These findings suggest that PHB01 has a potential use in therapy against infections caused by P. multocida type D. MDPI 2019-01-21 /pmc/articles/PMC6356340/ /pubmed/30669600 http://dx.doi.org/10.3390/v11010086 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yibao Guo, Guanghao Sun, Erchao Song, Jiaoyang Yang, Lan Zhu, Lili Liang, Wan Hua, Lin Peng, Zhong Tang, Xibiao Chen, Huanchun Wu, Bin Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title | Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title_full | Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title_fullStr | Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title_full_unstemmed | Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title_short | Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections |
title_sort | isolation of a t7-like lytic pasteurella bacteriophage vb_pmup_phb01 and its potential use in therapy against pasteurella multocida infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356340/ https://www.ncbi.nlm.nih.gov/pubmed/30669600 http://dx.doi.org/10.3390/v11010086 |
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