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Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen

Collagen is widely used in drugs, biomaterials, foods, and cosmetics. By-products of the fishing industry are rich sources of collagen, which can be used as an alternative to collagen traditionally harvested from land mammals. However, commercial applications of fish-based collagen are limited by th...

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Autores principales: Chen, Junde, Li, Min, Yi, Ruizao, Bai, Kaikai, Wang, Guangyu, Tan, Ran, Sun, Shanshan, Xu, Nuohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356396/
https://www.ncbi.nlm.nih.gov/pubmed/30621157
http://dx.doi.org/10.3390/md17010025
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author Chen, Junde
Li, Min
Yi, Ruizao
Bai, Kaikai
Wang, Guangyu
Tan, Ran
Sun, Shanshan
Xu, Nuohua
author_facet Chen, Junde
Li, Min
Yi, Ruizao
Bai, Kaikai
Wang, Guangyu
Tan, Ran
Sun, Shanshan
Xu, Nuohua
author_sort Chen, Junde
collection PubMed
description Collagen is widely used in drugs, biomaterials, foods, and cosmetics. By-products of the fishing industry are rich sources of collagen, which can be used as an alternative to collagen traditionally harvested from land mammals. However, commercial applications of fish-based collagen are limited by the low efficiency, low productivity, and low sustainability of the extraction process. This study applied a new technique (electrodialysis) for the extraction of Takifugu flavidus skin collagen. We found electrodialysis to have better economic and environmental outcomes than traditional dialysis as it significantly reduced the purification time and wastewater (~95%) while maintaining high extraction yield (67.3 ± 1.3 g/100 g dry weight, p < 0.05). SDS-PAGE, amino acid composition analysis, and spectrophotometric characterization indicated that electrodialysis treatment retained the physicochemical properties of T. flavidus collagen. Heavy metals and tetrodotoxin analyses indicated the safety of T. flavidus collagen. Notably, the collagen had similar thermal stability to calf skin collagen, with the maximum transition temperature and denaturation temperature of 41.8 ± 0.35 and 28.4 ± 2.5 °C, respectively. All evidence suggests that electrodialysis is a promising technique for extracting collagen in the fishing industry and that T. flavidus skin collagen could serve as an alternative source of collagen to meet the increasing demand from consumers.
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spelling pubmed-63563962019-02-05 Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen Chen, Junde Li, Min Yi, Ruizao Bai, Kaikai Wang, Guangyu Tan, Ran Sun, Shanshan Xu, Nuohua Mar Drugs Article Collagen is widely used in drugs, biomaterials, foods, and cosmetics. By-products of the fishing industry are rich sources of collagen, which can be used as an alternative to collagen traditionally harvested from land mammals. However, commercial applications of fish-based collagen are limited by the low efficiency, low productivity, and low sustainability of the extraction process. This study applied a new technique (electrodialysis) for the extraction of Takifugu flavidus skin collagen. We found electrodialysis to have better economic and environmental outcomes than traditional dialysis as it significantly reduced the purification time and wastewater (~95%) while maintaining high extraction yield (67.3 ± 1.3 g/100 g dry weight, p < 0.05). SDS-PAGE, amino acid composition analysis, and spectrophotometric characterization indicated that electrodialysis treatment retained the physicochemical properties of T. flavidus collagen. Heavy metals and tetrodotoxin analyses indicated the safety of T. flavidus collagen. Notably, the collagen had similar thermal stability to calf skin collagen, with the maximum transition temperature and denaturation temperature of 41.8 ± 0.35 and 28.4 ± 2.5 °C, respectively. All evidence suggests that electrodialysis is a promising technique for extracting collagen in the fishing industry and that T. flavidus skin collagen could serve as an alternative source of collagen to meet the increasing demand from consumers. MDPI 2019-01-04 /pmc/articles/PMC6356396/ /pubmed/30621157 http://dx.doi.org/10.3390/md17010025 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Junde
Li, Min
Yi, Ruizao
Bai, Kaikai
Wang, Guangyu
Tan, Ran
Sun, Shanshan
Xu, Nuohua
Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title_full Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title_fullStr Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title_full_unstemmed Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title_short Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen
title_sort electrodialysis extraction of pufferfish skin (takifugu flavidus): a promising source of collagen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356396/
https://www.ncbi.nlm.nih.gov/pubmed/30621157
http://dx.doi.org/10.3390/md17010025
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