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Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography

Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experim...

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Autores principales: Tsai, Meng-Tsan, Huang, Bo-Huei, Yeh, Chun-Chih, Lei, Kin Fong, Tsang, Ngan-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356435/
https://www.ncbi.nlm.nih.gov/pubmed/30621072
http://dx.doi.org/10.3390/mi10010035
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author Tsai, Meng-Tsan
Huang, Bo-Huei
Yeh, Chun-Chih
Lei, Kin Fong
Tsang, Ngan-Ming
author_facet Tsai, Meng-Tsan
Huang, Bo-Huei
Yeh, Chun-Chih
Lei, Kin Fong
Tsang, Ngan-Ming
author_sort Tsai, Meng-Tsan
collection PubMed
description Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experiment for characterizing the tumor development in vitro. However, quantification of the growth of cell colonies under a microscope is difficult because they are suspended in a three-dimensional environment. Thus, optical coherence tomography (OCT) imaging was develop in this study to monitor the growth of cell colonies. Cancer cell line of Huh 7 was used and the cells were applied on a layer of agarose hydrogel, i.e., a non-adherent surface. Then, cell colonies were gradually formed on the surface. The OCT technique was used to scan the cell colonies every day to obtain quantitative data for describing their growth. The results revealed the average volume increased with time due to the formation of cell colonies day-by-day. Additionally, the distribution of cell colony volume was analyzed to show the detailed information of the growth of the cell colonies. In summary, the OCT provides a non-invasive quantification technique for monitoring the growth of the cell colonies. From the OCT images, objective and precise information is obtained for higher prediction of the in vivo tumor development.
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spelling pubmed-63564352019-02-05 Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography Tsai, Meng-Tsan Huang, Bo-Huei Yeh, Chun-Chih Lei, Kin Fong Tsang, Ngan-Ming Micromachines (Basel) Article Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experiment for characterizing the tumor development in vitro. However, quantification of the growth of cell colonies under a microscope is difficult because they are suspended in a three-dimensional environment. Thus, optical coherence tomography (OCT) imaging was develop in this study to monitor the growth of cell colonies. Cancer cell line of Huh 7 was used and the cells were applied on a layer of agarose hydrogel, i.e., a non-adherent surface. Then, cell colonies were gradually formed on the surface. The OCT technique was used to scan the cell colonies every day to obtain quantitative data for describing their growth. The results revealed the average volume increased with time due to the formation of cell colonies day-by-day. Additionally, the distribution of cell colony volume was analyzed to show the detailed information of the growth of the cell colonies. In summary, the OCT provides a non-invasive quantification technique for monitoring the growth of the cell colonies. From the OCT images, objective and precise information is obtained for higher prediction of the in vivo tumor development. MDPI 2019-01-07 /pmc/articles/PMC6356435/ /pubmed/30621072 http://dx.doi.org/10.3390/mi10010035 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Meng-Tsan
Huang, Bo-Huei
Yeh, Chun-Chih
Lei, Kin Fong
Tsang, Ngan-Ming
Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title_full Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title_fullStr Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title_full_unstemmed Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title_short Non-Invasive Quantification of the Growth of Cancer Cell Colonies by a Portable Optical Coherence Tomography
title_sort non-invasive quantification of the growth of cancer cell colonies by a portable optical coherence tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356435/
https://www.ncbi.nlm.nih.gov/pubmed/30621072
http://dx.doi.org/10.3390/mi10010035
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