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UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356472/ https://www.ncbi.nlm.nih.gov/pubmed/30586934 http://dx.doi.org/10.3390/md17010010 |
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author | Nuzzo, Genoveffa Gallo, Carmela d’Ippolito, Giuliana Manzo, Emiliano Ruocco, Nadia Russo, Ennio Carotenuto, Ylenia Costantini, Maria Zupo, Valerio Sardo, Angela Fontana, Angelo |
author_facet | Nuzzo, Genoveffa Gallo, Carmela d’Ippolito, Giuliana Manzo, Emiliano Ruocco, Nadia Russo, Ennio Carotenuto, Ylenia Costantini, Maria Zupo, Valerio Sardo, Angela Fontana, Angelo |
author_sort | Nuzzo, Genoveffa |
collection | PubMed |
description | Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and molecular biology of diatoms is envisaged as a key step to understanding regulation of their life cycle in marine environments as well as facilitating their full and profitable exploitation by biotechnological platforms. Recently, we identified sterol sulfates (StS) as regulatory molecules of cell death in the diatom Skeletonema marinoi. As these compounds may have a general role in diatom physiology and chemical signals in aquatic systems, we investigated a suitable tool for their analysis in laboratory and field samples. Herein, we describe a sensitive, fast, and efficient ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method for qualitative and quantitative analysis of StS from crude extract of diatoms and other microalgae. The method was applied to 13 different strains of our collection of marine protists. This first study suggested a species-specific distribution of StS and identified the sulfated derivatives of 24-methylene cholesterol and 24-methyl cholesterol as the most common members in diatoms. |
format | Online Article Text |
id | pubmed-6356472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63564722019-02-05 UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms Nuzzo, Genoveffa Gallo, Carmela d’Ippolito, Giuliana Manzo, Emiliano Ruocco, Nadia Russo, Ennio Carotenuto, Ylenia Costantini, Maria Zupo, Valerio Sardo, Angela Fontana, Angelo Mar Drugs Article Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and molecular biology of diatoms is envisaged as a key step to understanding regulation of their life cycle in marine environments as well as facilitating their full and profitable exploitation by biotechnological platforms. Recently, we identified sterol sulfates (StS) as regulatory molecules of cell death in the diatom Skeletonema marinoi. As these compounds may have a general role in diatom physiology and chemical signals in aquatic systems, we investigated a suitable tool for their analysis in laboratory and field samples. Herein, we describe a sensitive, fast, and efficient ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method for qualitative and quantitative analysis of StS from crude extract of diatoms and other microalgae. The method was applied to 13 different strains of our collection of marine protists. This first study suggested a species-specific distribution of StS and identified the sulfated derivatives of 24-methylene cholesterol and 24-methyl cholesterol as the most common members in diatoms. MDPI 2018-12-24 /pmc/articles/PMC6356472/ /pubmed/30586934 http://dx.doi.org/10.3390/md17010010 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nuzzo, Genoveffa Gallo, Carmela d’Ippolito, Giuliana Manzo, Emiliano Ruocco, Nadia Russo, Ennio Carotenuto, Ylenia Costantini, Maria Zupo, Valerio Sardo, Angela Fontana, Angelo UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title | UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title_full | UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title_fullStr | UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title_full_unstemmed | UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title_short | UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms |
title_sort | uplc–ms/ms identification of sterol sulfates in marine diatoms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356472/ https://www.ncbi.nlm.nih.gov/pubmed/30586934 http://dx.doi.org/10.3390/md17010010 |
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