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UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms

Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and...

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Autores principales: Nuzzo, Genoveffa, Gallo, Carmela, d’Ippolito, Giuliana, Manzo, Emiliano, Ruocco, Nadia, Russo, Ennio, Carotenuto, Ylenia, Costantini, Maria, Zupo, Valerio, Sardo, Angela, Fontana, Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356472/
https://www.ncbi.nlm.nih.gov/pubmed/30586934
http://dx.doi.org/10.3390/md17010010
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author Nuzzo, Genoveffa
Gallo, Carmela
d’Ippolito, Giuliana
Manzo, Emiliano
Ruocco, Nadia
Russo, Ennio
Carotenuto, Ylenia
Costantini, Maria
Zupo, Valerio
Sardo, Angela
Fontana, Angelo
author_facet Nuzzo, Genoveffa
Gallo, Carmela
d’Ippolito, Giuliana
Manzo, Emiliano
Ruocco, Nadia
Russo, Ennio
Carotenuto, Ylenia
Costantini, Maria
Zupo, Valerio
Sardo, Angela
Fontana, Angelo
author_sort Nuzzo, Genoveffa
collection PubMed
description Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and molecular biology of diatoms is envisaged as a key step to understanding regulation of their life cycle in marine environments as well as facilitating their full and profitable exploitation by biotechnological platforms. Recently, we identified sterol sulfates (StS) as regulatory molecules of cell death in the diatom Skeletonema marinoi. As these compounds may have a general role in diatom physiology and chemical signals in aquatic systems, we investigated a suitable tool for their analysis in laboratory and field samples. Herein, we describe a sensitive, fast, and efficient ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method for qualitative and quantitative analysis of StS from crude extract of diatoms and other microalgae. The method was applied to 13 different strains of our collection of marine protists. This first study suggested a species-specific distribution of StS and identified the sulfated derivatives of 24-methylene cholesterol and 24-methyl cholesterol as the most common members in diatoms.
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spelling pubmed-63564722019-02-05 UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms Nuzzo, Genoveffa Gallo, Carmela d’Ippolito, Giuliana Manzo, Emiliano Ruocco, Nadia Russo, Ennio Carotenuto, Ylenia Costantini, Maria Zupo, Valerio Sardo, Angela Fontana, Angelo Mar Drugs Article Diatoms are unicellular eukaryotic organisms that play a key ecological and biogeochemical role in oceans as major primary producers. Recently, these microalgae have also attracted interest as a promising source of functional products with widespread relevance. Progress in the knowledge of cell and molecular biology of diatoms is envisaged as a key step to understanding regulation of their life cycle in marine environments as well as facilitating their full and profitable exploitation by biotechnological platforms. Recently, we identified sterol sulfates (StS) as regulatory molecules of cell death in the diatom Skeletonema marinoi. As these compounds may have a general role in diatom physiology and chemical signals in aquatic systems, we investigated a suitable tool for their analysis in laboratory and field samples. Herein, we describe a sensitive, fast, and efficient ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method for qualitative and quantitative analysis of StS from crude extract of diatoms and other microalgae. The method was applied to 13 different strains of our collection of marine protists. This first study suggested a species-specific distribution of StS and identified the sulfated derivatives of 24-methylene cholesterol and 24-methyl cholesterol as the most common members in diatoms. MDPI 2018-12-24 /pmc/articles/PMC6356472/ /pubmed/30586934 http://dx.doi.org/10.3390/md17010010 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nuzzo, Genoveffa
Gallo, Carmela
d’Ippolito, Giuliana
Manzo, Emiliano
Ruocco, Nadia
Russo, Ennio
Carotenuto, Ylenia
Costantini, Maria
Zupo, Valerio
Sardo, Angela
Fontana, Angelo
UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title_full UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title_fullStr UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title_full_unstemmed UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title_short UPLC–MS/MS Identification of Sterol Sulfates in Marine Diatoms
title_sort uplc–ms/ms identification of sterol sulfates in marine diatoms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356472/
https://www.ncbi.nlm.nih.gov/pubmed/30586934
http://dx.doi.org/10.3390/md17010010
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