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Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus
Heme oxygenase-1 (HO-1), a rate-limiting enzyme involved in the degradation of heme, is induced in response to a wide range of stress conditions. HO-1 exerts antiviral activity against a broad range of viruses, including the Hepatitis C virus, the human immunodeficiency virus, and the dengue virus b...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356520/ https://www.ncbi.nlm.nih.gov/pubmed/30577437 http://dx.doi.org/10.3390/v11010002 |
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author | El Kalamouni, Chaker Frumence, Etienne Bos, Sandra Turpin, Jonathan Nativel, Brice Harrabi, Wissal Wilkinson, David A. Meilhac, Olivier Gadea, Gilles Desprès, Philippe Krejbich-Trotot, Pascale Viranaïcken, Wildriss |
author_facet | El Kalamouni, Chaker Frumence, Etienne Bos, Sandra Turpin, Jonathan Nativel, Brice Harrabi, Wissal Wilkinson, David A. Meilhac, Olivier Gadea, Gilles Desprès, Philippe Krejbich-Trotot, Pascale Viranaïcken, Wildriss |
author_sort | El Kalamouni, Chaker |
collection | PubMed |
description | Heme oxygenase-1 (HO-1), a rate-limiting enzyme involved in the degradation of heme, is induced in response to a wide range of stress conditions. HO-1 exerts antiviral activity against a broad range of viruses, including the Hepatitis C virus, the human immunodeficiency virus, and the dengue virus by inhibiting viral growth. It has been reported that HO-1 displays antiviral activity against the Zika virus (ZIKV) but the mechanisms of viral inhibition remain largely unknown. Using a ZIKV RNA replicon with the Green Fluorescent Protein (GFP) as a reporter protein, we were able to show that HO-1 expression resulted in the inhibition of viral RNA replication. Conversely, we observed a decrease in HO-1 expression in cells replicating the ZIKV RNA replicon. The study of human cells infected with ZIKV showed that the HO-1 expression level was significantly lower once viral replication was established, thereby limiting the antiviral effect of HO-1. Our work highlights the capacity of ZIKV to thwart the anti-replicative activity of HO-1 in human cells. Therefore, the modulation of HO-1 as a novel therapeutic strategy against ZIKV infection may display limited effect. |
format | Online Article Text |
id | pubmed-6356520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63565202019-02-05 Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus El Kalamouni, Chaker Frumence, Etienne Bos, Sandra Turpin, Jonathan Nativel, Brice Harrabi, Wissal Wilkinson, David A. Meilhac, Olivier Gadea, Gilles Desprès, Philippe Krejbich-Trotot, Pascale Viranaïcken, Wildriss Viruses Article Heme oxygenase-1 (HO-1), a rate-limiting enzyme involved in the degradation of heme, is induced in response to a wide range of stress conditions. HO-1 exerts antiviral activity against a broad range of viruses, including the Hepatitis C virus, the human immunodeficiency virus, and the dengue virus by inhibiting viral growth. It has been reported that HO-1 displays antiviral activity against the Zika virus (ZIKV) but the mechanisms of viral inhibition remain largely unknown. Using a ZIKV RNA replicon with the Green Fluorescent Protein (GFP) as a reporter protein, we were able to show that HO-1 expression resulted in the inhibition of viral RNA replication. Conversely, we observed a decrease in HO-1 expression in cells replicating the ZIKV RNA replicon. The study of human cells infected with ZIKV showed that the HO-1 expression level was significantly lower once viral replication was established, thereby limiting the antiviral effect of HO-1. Our work highlights the capacity of ZIKV to thwart the anti-replicative activity of HO-1 in human cells. Therefore, the modulation of HO-1 as a novel therapeutic strategy against ZIKV infection may display limited effect. MDPI 2018-12-20 /pmc/articles/PMC6356520/ /pubmed/30577437 http://dx.doi.org/10.3390/v11010002 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article El Kalamouni, Chaker Frumence, Etienne Bos, Sandra Turpin, Jonathan Nativel, Brice Harrabi, Wissal Wilkinson, David A. Meilhac, Olivier Gadea, Gilles Desprès, Philippe Krejbich-Trotot, Pascale Viranaïcken, Wildriss Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title | Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title_full | Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title_fullStr | Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title_full_unstemmed | Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title_short | Subversion of the Heme Oxygenase-1 Antiviral Activity by Zika Virus |
title_sort | subversion of the heme oxygenase-1 antiviral activity by zika virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356520/ https://www.ncbi.nlm.nih.gov/pubmed/30577437 http://dx.doi.org/10.3390/v11010002 |
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