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The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors
The GH3 gene family plays a vital role in the phytohormone-related growth and developmental processes. The effects of allopolyploidization on GH3 gene structures and expression levels have not been reported. In this study, a total of 38, 25, and 66 GH3 genes were identified in Brassica rapa (A(r)A(r...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356818/ https://www.ncbi.nlm.nih.gov/pubmed/30658516 http://dx.doi.org/10.3390/genes10010058 |
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author | Wang, Ruihua Li, Mengdi Wu, Xiaoming Wang, Jianbo |
author_facet | Wang, Ruihua Li, Mengdi Wu, Xiaoming Wang, Jianbo |
author_sort | Wang, Ruihua |
collection | PubMed |
description | The GH3 gene family plays a vital role in the phytohormone-related growth and developmental processes. The effects of allopolyploidization on GH3 gene structures and expression levels have not been reported. In this study, a total of 38, 25, and 66 GH3 genes were identified in Brassica rapa (A(r)A(r)), Brassica oleracea (C(o)C(o)), and Brassica napus (A(n)AC(n)C(n)), respectively. BnaGH3 genes were unevenly distributed on chromosomes with 39 on A(n) and 27 on C(n), in which six BnaGH3 genes may appear as new genes. The whole genome triplication allowed the GH3 gene family to expand in diploid ancestors, and allopolyploidization made the GH3 gene family re-expand in B. napus. For most BnaGH3 genes, the exon-intron compositions were similar to diploid ancestors, while the cis-element distributions were obviously different from its ancestors. After allopolyploidization, the expression patterns of GH3 genes from ancestor species changed greatly in B. napus, and the orthologous gene pairs between A(n)/A(r) and C(n)/C(o) had diverged expression patterns across four tissues. Our study provides a comprehensive analysis of the GH3 gene family in B. napus, and these results could contribute to identifying genes with vital roles in phytohormone-related growth and developmental processes. |
format | Online Article Text |
id | pubmed-6356818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63568182019-02-04 The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors Wang, Ruihua Li, Mengdi Wu, Xiaoming Wang, Jianbo Genes (Basel) Article The GH3 gene family plays a vital role in the phytohormone-related growth and developmental processes. The effects of allopolyploidization on GH3 gene structures and expression levels have not been reported. In this study, a total of 38, 25, and 66 GH3 genes were identified in Brassica rapa (A(r)A(r)), Brassica oleracea (C(o)C(o)), and Brassica napus (A(n)AC(n)C(n)), respectively. BnaGH3 genes were unevenly distributed on chromosomes with 39 on A(n) and 27 on C(n), in which six BnaGH3 genes may appear as new genes. The whole genome triplication allowed the GH3 gene family to expand in diploid ancestors, and allopolyploidization made the GH3 gene family re-expand in B. napus. For most BnaGH3 genes, the exon-intron compositions were similar to diploid ancestors, while the cis-element distributions were obviously different from its ancestors. After allopolyploidization, the expression patterns of GH3 genes from ancestor species changed greatly in B. napus, and the orthologous gene pairs between A(n)/A(r) and C(n)/C(o) had diverged expression patterns across four tissues. Our study provides a comprehensive analysis of the GH3 gene family in B. napus, and these results could contribute to identifying genes with vital roles in phytohormone-related growth and developmental processes. MDPI 2019-01-17 /pmc/articles/PMC6356818/ /pubmed/30658516 http://dx.doi.org/10.3390/genes10010058 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Ruihua Li, Mengdi Wu, Xiaoming Wang, Jianbo The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title | The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title_full | The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title_fullStr | The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title_full_unstemmed | The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title_short | The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors |
title_sort | gene structure and expression level changes of the gh3 gene family in brassica napus relative to its diploid ancestors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356818/ https://www.ncbi.nlm.nih.gov/pubmed/30658516 http://dx.doi.org/10.3390/genes10010058 |
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