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Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susce...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356941/ https://www.ncbi.nlm.nih.gov/pubmed/30634469 http://dx.doi.org/10.3390/cells8010033 |
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author | Maillard, Florie Vazeille, Emilie Sauvanet, Pierre Sirvent, Pascal Bonnet, Richard Combaret, Lydie Chausse, Pierre Chevarin, Caroline Otero, Yolanda Fernandez Delcros, Geoffrey Chavanelle, Vivien Boisseau, Nathalie Barnich, Nicolas |
author_facet | Maillard, Florie Vazeille, Emilie Sauvanet, Pierre Sirvent, Pascal Bonnet, Richard Combaret, Lydie Chausse, Pierre Chevarin, Caroline Otero, Yolanda Fernandez Delcros, Geoffrey Chavanelle, Vivien Boisseau, Nathalie Barnich, Nicolas |
author_sort | Maillard, Florie |
collection | PubMed |
description | Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susceptibility. Thirty-five CEABAC10 male mice performed spontaneous PA (wheel group; n = 24) or not (controls; n = 11) for 12 weeks. At week 12, mice were orally challenged with the AIEC LF82 strain for 6 days. Body composition, glycaemic control, intestinal permeability, gut microbiota composition, and fecal short-chain fatty acids were assessed in both groups. Animals were fed a high fat/high sugar diet throughout the study. After exposure to AIEC, mesenteric adipose tissue weight was lower in the wheel group. Tight junction proteins expression increased with spontaneous PA, whereas systemic lipopolysaccharides were negatively correlated with the covered distance. Bifidobacterium and Lactobacillus decreased in controls, whereas Oscillospira and Ruminococcus increased in the wheel group. Fecal propionate and butyrate were also higher in the wheel group. In conclusion, spontaneous physical activity promotes healthy gut microbiota composition changes and increases short-chain fatty acids in CEABAC10 mice fed a Western diet and exposed to AIEC to mimic Crohn’s disease. |
format | Online Article Text |
id | pubmed-6356941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63569412019-02-06 Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility Maillard, Florie Vazeille, Emilie Sauvanet, Pierre Sirvent, Pascal Bonnet, Richard Combaret, Lydie Chausse, Pierre Chevarin, Caroline Otero, Yolanda Fernandez Delcros, Geoffrey Chavanelle, Vivien Boisseau, Nathalie Barnich, Nicolas Cells Article Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susceptibility. Thirty-five CEABAC10 male mice performed spontaneous PA (wheel group; n = 24) or not (controls; n = 11) for 12 weeks. At week 12, mice were orally challenged with the AIEC LF82 strain for 6 days. Body composition, glycaemic control, intestinal permeability, gut microbiota composition, and fecal short-chain fatty acids were assessed in both groups. Animals were fed a high fat/high sugar diet throughout the study. After exposure to AIEC, mesenteric adipose tissue weight was lower in the wheel group. Tight junction proteins expression increased with spontaneous PA, whereas systemic lipopolysaccharides were negatively correlated with the covered distance. Bifidobacterium and Lactobacillus decreased in controls, whereas Oscillospira and Ruminococcus increased in the wheel group. Fecal propionate and butyrate were also higher in the wheel group. In conclusion, spontaneous physical activity promotes healthy gut microbiota composition changes and increases short-chain fatty acids in CEABAC10 mice fed a Western diet and exposed to AIEC to mimic Crohn’s disease. MDPI 2019-01-09 /pmc/articles/PMC6356941/ /pubmed/30634469 http://dx.doi.org/10.3390/cells8010033 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Maillard, Florie Vazeille, Emilie Sauvanet, Pierre Sirvent, Pascal Bonnet, Richard Combaret, Lydie Chausse, Pierre Chevarin, Caroline Otero, Yolanda Fernandez Delcros, Geoffrey Chavanelle, Vivien Boisseau, Nathalie Barnich, Nicolas Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title | Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title_full | Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title_fullStr | Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title_full_unstemmed | Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title_short | Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility |
title_sort | preventive effect of spontaneous physical activity on the gut-adipose tissue in a mouse model that mimics crohn’s disease susceptibility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356941/ https://www.ncbi.nlm.nih.gov/pubmed/30634469 http://dx.doi.org/10.3390/cells8010033 |
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