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Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility

Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susce...

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Autores principales: Maillard, Florie, Vazeille, Emilie, Sauvanet, Pierre, Sirvent, Pascal, Bonnet, Richard, Combaret, Lydie, Chausse, Pierre, Chevarin, Caroline, Otero, Yolanda Fernandez, Delcros, Geoffrey, Chavanelle, Vivien, Boisseau, Nathalie, Barnich, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356941/
https://www.ncbi.nlm.nih.gov/pubmed/30634469
http://dx.doi.org/10.3390/cells8010033
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author Maillard, Florie
Vazeille, Emilie
Sauvanet, Pierre
Sirvent, Pascal
Bonnet, Richard
Combaret, Lydie
Chausse, Pierre
Chevarin, Caroline
Otero, Yolanda Fernandez
Delcros, Geoffrey
Chavanelle, Vivien
Boisseau, Nathalie
Barnich, Nicolas
author_facet Maillard, Florie
Vazeille, Emilie
Sauvanet, Pierre
Sirvent, Pascal
Bonnet, Richard
Combaret, Lydie
Chausse, Pierre
Chevarin, Caroline
Otero, Yolanda Fernandez
Delcros, Geoffrey
Chavanelle, Vivien
Boisseau, Nathalie
Barnich, Nicolas
author_sort Maillard, Florie
collection PubMed
description Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susceptibility. Thirty-five CEABAC10 male mice performed spontaneous PA (wheel group; n = 24) or not (controls; n = 11) for 12 weeks. At week 12, mice were orally challenged with the AIEC LF82 strain for 6 days. Body composition, glycaemic control, intestinal permeability, gut microbiota composition, and fecal short-chain fatty acids were assessed in both groups. Animals were fed a high fat/high sugar diet throughout the study. After exposure to AIEC, mesenteric adipose tissue weight was lower in the wheel group. Tight junction proteins expression increased with spontaneous PA, whereas systemic lipopolysaccharides were negatively correlated with the covered distance. Bifidobacterium and Lactobacillus decreased in controls, whereas Oscillospira and Ruminococcus increased in the wheel group. Fecal propionate and butyrate were also higher in the wheel group. In conclusion, spontaneous physical activity promotes healthy gut microbiota composition changes and increases short-chain fatty acids in CEABAC10 mice fed a Western diet and exposed to AIEC to mimic Crohn’s disease.
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spelling pubmed-63569412019-02-06 Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility Maillard, Florie Vazeille, Emilie Sauvanet, Pierre Sirvent, Pascal Bonnet, Richard Combaret, Lydie Chausse, Pierre Chevarin, Caroline Otero, Yolanda Fernandez Delcros, Geoffrey Chavanelle, Vivien Boisseau, Nathalie Barnich, Nicolas Cells Article Crohn’s disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn’s disease susceptibility. Thirty-five CEABAC10 male mice performed spontaneous PA (wheel group; n = 24) or not (controls; n = 11) for 12 weeks. At week 12, mice were orally challenged with the AIEC LF82 strain for 6 days. Body composition, glycaemic control, intestinal permeability, gut microbiota composition, and fecal short-chain fatty acids were assessed in both groups. Animals were fed a high fat/high sugar diet throughout the study. After exposure to AIEC, mesenteric adipose tissue weight was lower in the wheel group. Tight junction proteins expression increased with spontaneous PA, whereas systemic lipopolysaccharides were negatively correlated with the covered distance. Bifidobacterium and Lactobacillus decreased in controls, whereas Oscillospira and Ruminococcus increased in the wheel group. Fecal propionate and butyrate were also higher in the wheel group. In conclusion, spontaneous physical activity promotes healthy gut microbiota composition changes and increases short-chain fatty acids in CEABAC10 mice fed a Western diet and exposed to AIEC to mimic Crohn’s disease. MDPI 2019-01-09 /pmc/articles/PMC6356941/ /pubmed/30634469 http://dx.doi.org/10.3390/cells8010033 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maillard, Florie
Vazeille, Emilie
Sauvanet, Pierre
Sirvent, Pascal
Bonnet, Richard
Combaret, Lydie
Chausse, Pierre
Chevarin, Caroline
Otero, Yolanda Fernandez
Delcros, Geoffrey
Chavanelle, Vivien
Boisseau, Nathalie
Barnich, Nicolas
Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title_full Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title_fullStr Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title_full_unstemmed Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title_short Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility
title_sort preventive effect of spontaneous physical activity on the gut-adipose tissue in a mouse model that mimics crohn’s disease susceptibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356941/
https://www.ncbi.nlm.nih.gov/pubmed/30634469
http://dx.doi.org/10.3390/cells8010033
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