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Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips

Circulating tumour cells (CTCs) are the metastatic precursors to distant disease in head and neck cancers (HNCs). Whilst the prognostic and predictive value of single CTCs have been well documented, the role of CTC clusters, which potentially have a higher metastatic capacity are limited. In this st...

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Autores principales: Kulasinghe, Arutha, Zhou, Jian, Kenny, Liz, Papautsky, Ian, Punyadeera, Chamindie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356955/
https://www.ncbi.nlm.nih.gov/pubmed/30646614
http://dx.doi.org/10.3390/cancers11010089
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author Kulasinghe, Arutha
Zhou, Jian
Kenny, Liz
Papautsky, Ian
Punyadeera, Chamindie
author_facet Kulasinghe, Arutha
Zhou, Jian
Kenny, Liz
Papautsky, Ian
Punyadeera, Chamindie
author_sort Kulasinghe, Arutha
collection PubMed
description Circulating tumour cells (CTCs) are the metastatic precursors to distant disease in head and neck cancers (HNCs). Whilst the prognostic and predictive value of single CTCs have been well documented, the role of CTC clusters, which potentially have a higher metastatic capacity are limited. In this study, the authors used a novel straight microfluidic chip to focus and capture CTCs. The chip offers high cell recoveries with clinically relevant numbers (10–500 cells/mL) without the need for further purification. Single CTCs were identified in 10/21 patient samples (range 2–24 CTCs/mL), CTC clusters in 9/21 patient samples (range 1–6 CTC clusters/mL) and circulating tumour microemboli (CTM) in 2/21 samples. This study demonstrated that CTC clusters contain EGFR amplified single CTCs within the cluster volume. This novel microfluidic chip demonstrates the efficient sorting and preservation of single CTCs, CTC clusters and CTMs. The authors intend to expand this study to a larger cohort to determine the clinical implication of the CTC subsets in HNC.
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spelling pubmed-63569552019-02-05 Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips Kulasinghe, Arutha Zhou, Jian Kenny, Liz Papautsky, Ian Punyadeera, Chamindie Cancers (Basel) Article Circulating tumour cells (CTCs) are the metastatic precursors to distant disease in head and neck cancers (HNCs). Whilst the prognostic and predictive value of single CTCs have been well documented, the role of CTC clusters, which potentially have a higher metastatic capacity are limited. In this study, the authors used a novel straight microfluidic chip to focus and capture CTCs. The chip offers high cell recoveries with clinically relevant numbers (10–500 cells/mL) without the need for further purification. Single CTCs were identified in 10/21 patient samples (range 2–24 CTCs/mL), CTC clusters in 9/21 patient samples (range 1–6 CTC clusters/mL) and circulating tumour microemboli (CTM) in 2/21 samples. This study demonstrated that CTC clusters contain EGFR amplified single CTCs within the cluster volume. This novel microfluidic chip demonstrates the efficient sorting and preservation of single CTCs, CTC clusters and CTMs. The authors intend to expand this study to a larger cohort to determine the clinical implication of the CTC subsets in HNC. MDPI 2019-01-14 /pmc/articles/PMC6356955/ /pubmed/30646614 http://dx.doi.org/10.3390/cancers11010089 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kulasinghe, Arutha
Zhou, Jian
Kenny, Liz
Papautsky, Ian
Punyadeera, Chamindie
Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title_full Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title_fullStr Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title_full_unstemmed Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title_short Capture of Circulating Tumour Cell Clusters Using Straight Microfluidic Chips
title_sort capture of circulating tumour cell clusters using straight microfluidic chips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356955/
https://www.ncbi.nlm.nih.gov/pubmed/30646614
http://dx.doi.org/10.3390/cancers11010089
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