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Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy

The HIV-1 assembly process is a multi-complex mechanism that takes place at the host cell plasma membrane. It requires a spatio-temporal coordination of events to end up with a full mature and infectious virus. The molecular mechanisms of HIV-1 assembly have been extensively studied during the past...

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Autores principales: Inamdar, Kaushik, Floderer, Charlotte, Favard, Cyril, Muriaux, Delphine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357049/
https://www.ncbi.nlm.nih.gov/pubmed/30654596
http://dx.doi.org/10.3390/v11010072
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author Inamdar, Kaushik
Floderer, Charlotte
Favard, Cyril
Muriaux, Delphine
author_facet Inamdar, Kaushik
Floderer, Charlotte
Favard, Cyril
Muriaux, Delphine
author_sort Inamdar, Kaushik
collection PubMed
description The HIV-1 assembly process is a multi-complex mechanism that takes place at the host cell plasma membrane. It requires a spatio-temporal coordination of events to end up with a full mature and infectious virus. The molecular mechanisms of HIV-1 assembly have been extensively studied during the past decades, in order to dissect the respective roles of the structural and non-structural viral proteins of the viral RNA genome and of some host cell factors. Nevertheless, the time course of HIV-1 assembly was observed in living cells only a decade ago. The very recent revolution of optical microscopy, combining high speed and high spatial resolution, in addition to improved fluorescent tags for proteins, now permits study of HIV-1 assembly at the single molecule level within living cells. In this review, after a short description of these new approaches, we will discuss how HIV-1 assembly at the cell plasma membrane has been revisited using advanced super resolution microscopy techniques and how it can bridge the study of viral assembly from the single molecule to the entire host cell.
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spelling pubmed-63570492019-02-05 Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy Inamdar, Kaushik Floderer, Charlotte Favard, Cyril Muriaux, Delphine Viruses Review The HIV-1 assembly process is a multi-complex mechanism that takes place at the host cell plasma membrane. It requires a spatio-temporal coordination of events to end up with a full mature and infectious virus. The molecular mechanisms of HIV-1 assembly have been extensively studied during the past decades, in order to dissect the respective roles of the structural and non-structural viral proteins of the viral RNA genome and of some host cell factors. Nevertheless, the time course of HIV-1 assembly was observed in living cells only a decade ago. The very recent revolution of optical microscopy, combining high speed and high spatial resolution, in addition to improved fluorescent tags for proteins, now permits study of HIV-1 assembly at the single molecule level within living cells. In this review, after a short description of these new approaches, we will discuss how HIV-1 assembly at the cell plasma membrane has been revisited using advanced super resolution microscopy techniques and how it can bridge the study of viral assembly from the single molecule to the entire host cell. MDPI 2019-01-16 /pmc/articles/PMC6357049/ /pubmed/30654596 http://dx.doi.org/10.3390/v11010072 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Inamdar, Kaushik
Floderer, Charlotte
Favard, Cyril
Muriaux, Delphine
Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title_full Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title_fullStr Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title_full_unstemmed Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title_short Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy
title_sort monitoring hiv-1 assembly in living cells: insights from dynamic and single molecule microscopy
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357049/
https://www.ncbi.nlm.nih.gov/pubmed/30654596
http://dx.doi.org/10.3390/v11010072
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