Cargando…

Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication

Lipids play numerous indispensable cellular functions and are involved in multiple steps in the replication cycle of viruses. Infections by human-pathogenic coronaviruses result in diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifes...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Bingpeng, Chu, Hin, Yang, Dong, Sze, Kong-Hung, Lai, Pok-Man, Yuan, Shuofeng, Shuai, Huiping, Wang, Yixin, Kao, Richard Yi-Tsun, Chan, Jasper Fuk-Woo, Yuen, Kwok-Yung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357182/
https://www.ncbi.nlm.nih.gov/pubmed/30654597
http://dx.doi.org/10.3390/v11010073
_version_ 1783391743625920512
author Yan, Bingpeng
Chu, Hin
Yang, Dong
Sze, Kong-Hung
Lai, Pok-Man
Yuan, Shuofeng
Shuai, Huiping
Wang, Yixin
Kao, Richard Yi-Tsun
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
author_facet Yan, Bingpeng
Chu, Hin
Yang, Dong
Sze, Kong-Hung
Lai, Pok-Man
Yuan, Shuofeng
Shuai, Huiping
Wang, Yixin
Kao, Richard Yi-Tsun
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
author_sort Yan, Bingpeng
collection PubMed
description Lipids play numerous indispensable cellular functions and are involved in multiple steps in the replication cycle of viruses. Infections by human-pathogenic coronaviruses result in diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how cellular lipids may modulate the pathogenicity of human-pathogenic coronaviruses remains poor. To this end, we utilized the human coronavirus 229E (HCoV-229E) as a model coronavirus to comprehensively characterize the host cell lipid response upon coronavirus infection with an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Our results revealed that glycerophospholipids and fatty acids (FAs) were significantly elevated in the HCoV-229E-infected cells and the linoleic acid (LA) to arachidonic acid (AA) metabolism axis was markedly perturbed upon HCoV-229E infection. Interestingly, exogenous supplement of LA or AA in HCoV-229E-infected cells significantly suppressed HCoV-229E virus replication. Importantly, the inhibitory effect of LA and AA on virus replication was also conserved for the highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV). Taken together, our study demonstrated that host lipid metabolic remodeling was significantly associated with human-pathogenic coronavirus propagation. Our data further suggested that lipid metabolism regulation would be a common and druggable target for coronavirus infections.
format Online
Article
Text
id pubmed-6357182
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63571822019-02-05 Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication Yan, Bingpeng Chu, Hin Yang, Dong Sze, Kong-Hung Lai, Pok-Man Yuan, Shuofeng Shuai, Huiping Wang, Yixin Kao, Richard Yi-Tsun Chan, Jasper Fuk-Woo Yuen, Kwok-Yung Viruses Article Lipids play numerous indispensable cellular functions and are involved in multiple steps in the replication cycle of viruses. Infections by human-pathogenic coronaviruses result in diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how cellular lipids may modulate the pathogenicity of human-pathogenic coronaviruses remains poor. To this end, we utilized the human coronavirus 229E (HCoV-229E) as a model coronavirus to comprehensively characterize the host cell lipid response upon coronavirus infection with an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Our results revealed that glycerophospholipids and fatty acids (FAs) were significantly elevated in the HCoV-229E-infected cells and the linoleic acid (LA) to arachidonic acid (AA) metabolism axis was markedly perturbed upon HCoV-229E infection. Interestingly, exogenous supplement of LA or AA in HCoV-229E-infected cells significantly suppressed HCoV-229E virus replication. Importantly, the inhibitory effect of LA and AA on virus replication was also conserved for the highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV). Taken together, our study demonstrated that host lipid metabolic remodeling was significantly associated with human-pathogenic coronavirus propagation. Our data further suggested that lipid metabolism regulation would be a common and druggable target for coronavirus infections. MDPI 2019-01-16 /pmc/articles/PMC6357182/ /pubmed/30654597 http://dx.doi.org/10.3390/v11010073 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Bingpeng
Chu, Hin
Yang, Dong
Sze, Kong-Hung
Lai, Pok-Man
Yuan, Shuofeng
Shuai, Huiping
Wang, Yixin
Kao, Richard Yi-Tsun
Chan, Jasper Fuk-Woo
Yuen, Kwok-Yung
Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title_full Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title_fullStr Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title_full_unstemmed Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title_short Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication
title_sort characterization of the lipidomic profile of human coronavirus-infected cells: implications for lipid metabolism remodeling upon coronavirus replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357182/
https://www.ncbi.nlm.nih.gov/pubmed/30654597
http://dx.doi.org/10.3390/v11010073
work_keys_str_mv AT yanbingpeng characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT chuhin characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT yangdong characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT szekonghung characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT laipokman characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT yuanshuofeng characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT shuaihuiping characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT wangyixin characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT kaorichardyitsun characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT chanjasperfukwoo characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication
AT yuenkwokyung characterizationofthelipidomicprofileofhumancoronavirusinfectedcellsimplicationsforlipidmetabolismremodelinguponcoronavirusreplication