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Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells

Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding...

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Autores principales: Frauz, Katleen, Teodoro, Luis Felipe R., Carneiro, Giane Daniela, Cristina da Veiga, Fernanda, Lopes Ferrucci, Danilo, Luis Bombeiro, André, Waleska Simões, Priscyla, Elvira Alvares, Lúcia, Leite R. de Oliveira, Alexandre, Pontes Vicente, Cristina, Seabra Ferreira, Rui, Barraviera, Benedito, do Amaral, Maria Esméria C., Augusto M. Esquisatto, Marcelo, de Campos Vidal, Benedicto, Rosa Pimentel, Edson, Aparecida de Aro, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357188/
https://www.ncbi.nlm.nih.gov/pubmed/30654437
http://dx.doi.org/10.3390/cells8010056
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author Frauz, Katleen
Teodoro, Luis Felipe R.
Carneiro, Giane Daniela
Cristina da Veiga, Fernanda
Lopes Ferrucci, Danilo
Luis Bombeiro, André
Waleska Simões, Priscyla
Elvira Alvares, Lúcia
Leite R. de Oliveira, Alexandre
Pontes Vicente, Cristina
Seabra Ferreira, Rui
Barraviera, Benedito
do Amaral, Maria Esméria C.
Augusto M. Esquisatto, Marcelo
de Campos Vidal, Benedicto
Rosa Pimentel, Edson
Aparecida de Aro, Andrea
author_facet Frauz, Katleen
Teodoro, Luis Felipe R.
Carneiro, Giane Daniela
Cristina da Veiga, Fernanda
Lopes Ferrucci, Danilo
Luis Bombeiro, André
Waleska Simões, Priscyla
Elvira Alvares, Lúcia
Leite R. de Oliveira, Alexandre
Pontes Vicente, Cristina
Seabra Ferreira, Rui
Barraviera, Benedito
do Amaral, Maria Esméria C.
Augusto M. Esquisatto, Marcelo
de Campos Vidal, Benedicto
Rosa Pimentel, Edson
Aparecida de Aro, Andrea
author_sort Frauz, Katleen
collection PubMed
description Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC). The in vivo imaging showed higher quantification of transplanted PKH26-labeled ASC in tendons of FS + ASC compared to ASC on the 14th day after transection. A small number of Iba1 labeled macrophages carrying PKH26 signal, probably due to phagocytosis of dead ASC, were observed in tendons of transected groups. ASC up-regulated the Tenomodulin gene expression in the transection region when compared to N, T and FS groups and the expression of TIMP-2 and Scleraxis genes in relation to the N group. FS group presented a greater organization of collagen fibers, followed by FS + ASC and ASC in comparison to N. Tendons from ASC group presented higher hydroxyproline concentration in relation to N and the transected tendons of T, FS and FS + ASC had a higher amount of collagen I and tenomodulin in comparison to N group. Although no marked differences were observed in the other biomechanical parameters, T group had higher value of maximum load compared to the groups ASC and FS + ASC. In conclusion, the FS kept constant the number of transplanted ASC in the transected region until the 14th day after injury. Our data suggest this FS to be a good scaffold for treatment during tendon repair because it was the most effective one regarding tendon organization recovering, followed by the FS treatment associated with ASC and finally by the transplanted ASC on the 21st day. Further investigations in long-term time points of the tendon repair are needed to analyze if the higher tissue organization found with the FS scaffold will improve the biomechanics of the tendons.
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spelling pubmed-63571882019-02-06 Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells Frauz, Katleen Teodoro, Luis Felipe R. Carneiro, Giane Daniela Cristina da Veiga, Fernanda Lopes Ferrucci, Danilo Luis Bombeiro, André Waleska Simões, Priscyla Elvira Alvares, Lúcia Leite R. de Oliveira, Alexandre Pontes Vicente, Cristina Seabra Ferreira, Rui Barraviera, Benedito do Amaral, Maria Esméria C. Augusto M. Esquisatto, Marcelo de Campos Vidal, Benedicto Rosa Pimentel, Edson Aparecida de Aro, Andrea Cells Article Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC). The in vivo imaging showed higher quantification of transplanted PKH26-labeled ASC in tendons of FS + ASC compared to ASC on the 14th day after transection. A small number of Iba1 labeled macrophages carrying PKH26 signal, probably due to phagocytosis of dead ASC, were observed in tendons of transected groups. ASC up-regulated the Tenomodulin gene expression in the transection region when compared to N, T and FS groups and the expression of TIMP-2 and Scleraxis genes in relation to the N group. FS group presented a greater organization of collagen fibers, followed by FS + ASC and ASC in comparison to N. Tendons from ASC group presented higher hydroxyproline concentration in relation to N and the transected tendons of T, FS and FS + ASC had a higher amount of collagen I and tenomodulin in comparison to N group. Although no marked differences were observed in the other biomechanical parameters, T group had higher value of maximum load compared to the groups ASC and FS + ASC. In conclusion, the FS kept constant the number of transplanted ASC in the transected region until the 14th day after injury. Our data suggest this FS to be a good scaffold for treatment during tendon repair because it was the most effective one regarding tendon organization recovering, followed by the FS treatment associated with ASC and finally by the transplanted ASC on the 21st day. Further investigations in long-term time points of the tendon repair are needed to analyze if the higher tissue organization found with the FS scaffold will improve the biomechanics of the tendons. MDPI 2019-01-16 /pmc/articles/PMC6357188/ /pubmed/30654437 http://dx.doi.org/10.3390/cells8010056 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Frauz, Katleen
Teodoro, Luis Felipe R.
Carneiro, Giane Daniela
Cristina da Veiga, Fernanda
Lopes Ferrucci, Danilo
Luis Bombeiro, André
Waleska Simões, Priscyla
Elvira Alvares, Lúcia
Leite R. de Oliveira, Alexandre
Pontes Vicente, Cristina
Seabra Ferreira, Rui
Barraviera, Benedito
do Amaral, Maria Esméria C.
Augusto M. Esquisatto, Marcelo
de Campos Vidal, Benedicto
Rosa Pimentel, Edson
Aparecida de Aro, Andrea
Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title_full Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title_fullStr Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title_full_unstemmed Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title_short Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells
title_sort transected tendon treated with a new fibrin sealant alone or associated with adipose-derived stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357188/
https://www.ncbi.nlm.nih.gov/pubmed/30654437
http://dx.doi.org/10.3390/cells8010056
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