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Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures
Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357748/ https://www.ncbi.nlm.nih.gov/pubmed/30746468 http://dx.doi.org/10.1126/sciadv.aau9223 |
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author | Reyes, Miguel Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Blainey, Paul C. |
author_facet | Reyes, Miguel Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Blainey, Paul C. |
author_sort | Reyes, Miguel |
collection | PubMed |
description | Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings. |
format | Online Article Text |
id | pubmed-6357748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63577482019-02-11 Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures Reyes, Miguel Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Blainey, Paul C. Sci Adv Research Articles Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system’s technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings. American Association for the Advancement of Science 2019-01-23 /pmc/articles/PMC6357748/ /pubmed/30746468 http://dx.doi.org/10.1126/sciadv.aau9223 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Reyes, Miguel Vickers, Dwayne Billman, Kianna Eisenhaure, Thomas Hoover, Paul Browne, Edward P. Rao, Deepak A. Hacohen, Nir Blainey, Paul C. Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title_full | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title_fullStr | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title_full_unstemmed | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title_short | Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
title_sort | multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357748/ https://www.ncbi.nlm.nih.gov/pubmed/30746468 http://dx.doi.org/10.1126/sciadv.aau9223 |
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